Nano-in-Micro Smart Hydrogel Composite for a Rapid Sensitive Immunoassay

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

Detail(s)

Original languageEnglish
Article number1801277
Journal / PublicationAdvanced Healthcare Materials
Volume8
Issue number4
Online published23 Jan 2019
Publication statusPublished - 21 Feb 2019
Externally publishedYes

Abstract

Immunoassays are an important tool in various bioanalytical settings, such as clinical diagnostics, biopharmaceutical analysis, environmental monitoring, and food testing. An enzyme-linked immunosorbent assay (ELISA) is usually used to amplify immunoassay signals; however, it requires labor-intensive and time-consuming procedures, which hinders its application to rapid cytokine detection. In this study, a nano-in-micro composite system, where immunosensing polystyrene beads (≈320 nm) are incorporated within a stimuli-responsive microgel matrix (≈40 µm) via microfluidics, is investigated. The intrinsic volume phase-transition change properties of the smart microgels allows an enzyme-free enhanced immunoassay, enabling instant enhancement in signal-to-noise ratios of ≈5-fold. This nano-in-micro hydrogel composite offers a simple yet highly effective method for sensitive and multiplexed cytokine analysis without complex enzyme-based signal amplification steps, greatly benefitting advanced immune medicine.

Research Area(s)

  • droplet microfluidics, immunofluorescence assays, microgel immunosensors, signal enhancement

Citation Format(s)

Nano-in-Micro Smart Hydrogel Composite for a Rapid Sensitive Immunoassay. / Hsu, Myat Noe; Wei, Shih-Chung; Phan, Dinh-Tuan; Zhang, Yong; Chen, Chia-Hung.

In: Advanced Healthcare Materials, Vol. 8, No. 4, 1801277, 21.02.2019.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review