Nanoconfinement greatly speeds up the nucleation and the annealing in single-DNA collapse

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

3 Scopus Citations
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Author(s)

  • Liang Dai
  • Jeremy J. Jones
  • Alexander R. Klotz
  • Stephen Levy
  • Patrick S. Doyle

Detail(s)

Original languageEnglish
Pages (from-to)6363-6371
Journal / PublicationSoft Matter
Volume13
Issue number37
Online published29 Aug 2017
Publication statusPublished - 7 Oct 2017
Externally publishedYes

Abstract

Manipulating and measuring single-molecule dynamics and reactions in nanofluidics is a rapidly growing field with broad applications in developing new biotechnologies, understanding nanoconfinement effects in vivo, and exploring new phenomena in confinement. In this work, we investigate the kinetics of DNA collapse in nanoslits using single T4-DNA (165.6 kbp) and λ-DNA (48.5 kbp), with particular focus on the measurement of the nucleation and annealing times. Fixing the ethanol concentration at 35% and varying the slit height from 2000 to 31 nm, the nucleation time dramatically decreases from more than 1 hour to a few minutes or less. The increased collapsed rate results from the larger free energy experienced by coiled DNA in confinement relative to compacted DNA. Our results also shed light on other conformational transitions in confinement, such as protein folding.

Citation Format(s)

Nanoconfinement greatly speeds up the nucleation and the annealing in single-DNA collapse. / Dai, Liang; Jones, Jeremy J.; Klotz, Alexander R.; Levy, Stephen; Doyle, Patrick S.

In: Soft Matter, Vol. 13, No. 37, 07.10.2017, p. 6363-6371.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review