N6-Methyldeoxyadenosine Marks Active Transcription Start Sites in Chlamydomonas

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Ye Fu
  • Guan-Zheng Luo
  • Kai Chen
  • Miao Yu
  • Dali Han
  • Ziyang Hao
  • Jianzhao Liu
  • Xingyu Lu
  • Louis C. Doré
  • Xiaocheng Weng
  • Quanjiang Ji
  • Laurens Mets
  • Chuan He

Detail(s)

Original languageEnglish
Pages (from-to)879-892
Journal / PublicationCell
Volume161
Issue number4
Online published30 Apr 2015
Publication statusPublished - 7 May 2015
Externally publishedYes

Abstract

N6-methyldeoxyadenosine (6mA or m6A) is a DNA modification preserved in prokaryotes to eukaryotes. It is widespread in bacteria and functions in DNA mismatch repair, chromosome segregation, and virulence regulation. In contrast, the distribution and function of 6mA in eukaryotes have been unclear. Here, we present a comprehensive analysis of the 6mA landscape in the genome of Chlamydomonas using new sequencing approaches. We identified the 6mA modification in 84% of genes in Chlamydomonas. We found that 6mA mainly locates at ApT dinucleotides around transcription start sites (TSS) with a bimodal distribution and appears to mark active genes. A periodic pattern of 6mA deposition was also observed at base resolution, which is associated with nucleosome distribution near the TSS, suggesting a possible role in nucleosome positioning. The new genome-wide mapping of 6mA and its unique distribution in the Chlamydomonas genome suggest potential regulatory roles of 6mA in gene expression in eukaryotic organisms.

Citation Format(s)

N6-Methyldeoxyadenosine Marks Active Transcription Start Sites in Chlamydomonas. / Fu, Ye; Luo, Guan-Zheng; Chen, Kai; Deng, Xin; Yu, Miao; Han, Dali; Hao, Ziyang; Liu, Jianzhao; Lu, Xingyu; Doré, Louis C.; Weng, Xiaocheng; Ji, Quanjiang; Mets, Laurens; He, Chuan.

In: Cell, Vol. 161, No. 4, 07.05.2015, p. 879-892.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review