MXene-Based Aptameric Fluorosensor for Sensitive and Rapid Detection of COVID-19
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Author(s)
Related Research Unit(s)
Detail(s)
Original language | English |
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Article number | 2301146 |
Journal / Publication | Small |
Volume | 19 |
Issue number | 23 |
Online published | 6 Mar 2023 |
Publication status | Published - 7 Jun 2023 |
Link(s)
Abstract
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-caused COVID-19 pandemic has rapidly escalated into the largest global health emergency, which pushes to develop detection kits for the detection of COVID-19 with high sensitivity, specificity, and fast analysis. Here, aptamer-functionalized MXene nanosheet is demonstrated as a novel bionanosensor that detects COVID-19. Upon binding to the spike receptor binding domain of SARS-CoV-2, the aptamer probe is released from MXene surface restoring the quenched fluorescence. The performances of the fluorosensor are evaluated using antigen protein, cultured virus, and swab specimens from COVID-19 patients. It is evidenced that this sensor can detect SARS-CoV-2 spike protein at final concentration of 38.9 fg mL−1 and SARS-CoV-2 pseudovirus (limit of detection: 7.2 copies) within 30 min. Its application for clinical samples analysis is also demonstrated successfully. This work offers an effective sensing platform for sensitive and rapid detection of COVID-19 with high specificity. © 2023 Wiley-VCH GmbH.
Research Area(s)
- cellular imaging, COVID-19, fluorosensors, MXene nanosheets, spike proteins
Citation Format(s)
MXene-Based Aptameric Fluorosensor for Sensitive and Rapid Detection of COVID-19. / Luo, Yao; Jiang, Xin; Zhang, Rong et al.
In: Small, Vol. 19, No. 23, 2301146, 07.06.2023.
In: Small, Vol. 19, No. 23, 2301146, 07.06.2023.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review