MULTIPLEXED PROTEASE ACTIVITY ASSAY AND INHIBITOR ANALYSIS USING PICO-INJECTOR ARRAY

Ee Xien Ng; Miles A. Miller; Chia-Hung Chen

MULTIPLEXED PROTEASE ACTIVITY ASSAY AND INHIBITOR ANALYSIS USING PICO-INJECTOR ARRAY

Ee Xien Ng
, Miles A. Miller
, Chia-Hung Chen^*

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

Abstract

Proteolytic activity matrix analysis (PrAMA) is a technique for simultaneously measuring multiple specific protease activities, generally requiring multiple measurements of parallel enzyme reactions combined with inhibitor analyses, and the approach is generally limited by sample quantity and the complexity of multiple reaction monitoring. To address these issues, in this study we developed a pico-injector array to generate 72 different reactions in picoliter-sized droplets by controlling the sequence of combinational injections, which allows simultaneous read-outs of a wide range of multiple enzyme reactions and measurement of inhibitor effects by using small sample volumes.

Original language	English
Title of host publication	18th International Conference on Miniaturized Systems for Chemistry and Life Science (MicroTAS 2014)
Publisher	Chemical and Biological Microsystems Society
Pages	2550-2551
ISBN (Print)	978-1-63439-697-4
Publication status	Published - Oct 2014
Externally published	Yes
Event	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: 26 Oct 2014 → 30 Oct 2014

Conference

Conference	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Place	United States
City	San Antonio
Period	26/10/14 → 30/10/14

Research Keywords

Emulsions
Inhibitor analysis
Multiplexed bioassay
Pico-injector array
Proteases

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@inproceedings{a01b66a28217452aa8dca1f9c9bc9abe,

title = "MULTIPLEXED PROTEASE ACTIVITY ASSAY AND INHIBITOR ANALYSIS USING PICO-INJECTOR ARRAY",

abstract = "Proteolytic activity matrix analysis (PrAMA) is a technique for simultaneously measuring multiple specific protease activities, generally requiring multiple measurements of parallel enzyme reactions combined with inhibitor analyses, and the approach is generally limited by sample quantity and the complexity of multiple reaction monitoring. To address these issues, in this study we developed a pico-injector array to generate 72 different reactions in picoliter-sized droplets by controlling the sequence of combinational injections, which allows simultaneous read-outs of a wide range of multiple enzyme reactions and measurement of inhibitor effects by using small sample volumes.",

keywords = "Emulsions, Inhibitor analysis, Multiplexed bioassay, Pico-injector array, Proteases",

author = "Ng, \{Ee Xien\} and Miller, \{Miles A.\} and Chia-Hung Chen",

year = "2014",

month = oct,

language = "English",

isbn = "978-1-63439-697-4",

pages = "2550--2551",

booktitle = "18th International Conference on Miniaturized Systems for Chemistry and Life Science (MicroTAS 2014)",

publisher = "Chemical and Biological Microsystems Society",

note = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 ; Conference date: 26-10-2014 Through 30-10-2014",

}

Ng, EX, Miller, MA & Chen, C-H 2014, MULTIPLEXED PROTEASE ACTIVITY ASSAY AND INHIBITOR ANALYSIS USING PICO-INJECTOR ARRAY. in 18th International Conference on Miniaturized Systems for Chemistry and Life Science (MicroTAS 2014). Chemical and Biological Microsystems Society, pp. 2550-2551, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 26/10/14.

MULTIPLEXED PROTEASE ACTIVITY ASSAY AND INHIBITOR ANALYSIS USING PICO-INJECTOR ARRAY. / Ng, Ee Xien; Miller, Miles A.; Chen, Chia-Hung.
18th International Conference on Miniaturized Systems for Chemistry and Life Science (MicroTAS 2014). Chemical and Biological Microsystems Society, 2014. p. 2550-2551.

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - MULTIPLEXED PROTEASE ACTIVITY ASSAY AND INHIBITOR ANALYSIS USING PICO-INJECTOR ARRAY

AU - Ng, Ee Xien

AU - Miller, Miles A.

AU - Chen, Chia-Hung

PY - 2014/10

Y1 - 2014/10

N2 - Proteolytic activity matrix analysis (PrAMA) is a technique for simultaneously measuring multiple specific protease activities, generally requiring multiple measurements of parallel enzyme reactions combined with inhibitor analyses, and the approach is generally limited by sample quantity and the complexity of multiple reaction monitoring. To address these issues, in this study we developed a pico-injector array to generate 72 different reactions in picoliter-sized droplets by controlling the sequence of combinational injections, which allows simultaneous read-outs of a wide range of multiple enzyme reactions and measurement of inhibitor effects by using small sample volumes.

AB - Proteolytic activity matrix analysis (PrAMA) is a technique for simultaneously measuring multiple specific protease activities, generally requiring multiple measurements of parallel enzyme reactions combined with inhibitor analyses, and the approach is generally limited by sample quantity and the complexity of multiple reaction monitoring. To address these issues, in this study we developed a pico-injector array to generate 72 different reactions in picoliter-sized droplets by controlling the sequence of combinational injections, which allows simultaneous read-outs of a wide range of multiple enzyme reactions and measurement of inhibitor effects by using small sample volumes.

KW - Emulsions

KW - Inhibitor analysis

KW - Multiplexed bioassay

KW - Pico-injector array

KW - Proteases

UR - https://www.scopus.com/pages/publications/84941670658

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84941670658&origin=recordpage

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 978-1-63439-697-4

SP - 2550

EP - 2551

BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Science (MicroTAS 2014)

PB - Chemical and Biological Microsystems Society

T2 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Y2 - 26 October 2014 through 30 October 2014

ER -

MULTIPLEXED PROTEASE ACTIVITY ASSAY AND INHIBITOR ANALYSIS USING PICO-INJECTOR ARRAY

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Research Keywords

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