Multiplex single-nucleotide polymorphism typing by nanoparticle-coupled DNA-templated reactions

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

72 Scopus Citations
View graph of relations

Author(s)

Detail(s)

Original languageEnglish
Pages (from-to)11668-11669
Journal / PublicationJournal of the American Chemical Society
Volume131
Issue number33
Publication statusPublished - 26 Aug 2009
Externally publishedYes

Abstract

(Figure Presented) A novel chip-based detection approach for single-nucleotide polymorphism (SNP) typing based on nanoparticle-coupled DNA-templated ligation reactions is reported. In contrast to conventional methods or recently developed techniques, this approach does not need costly instrumentation and complex stringency washing processes and offers both rapid multiplex SNP detection capability and ultrahigh sensitivity. The ability of the approach to quickly identify the precise location of the single-base mismatch may provide a time-efficient method for high-throughput multiplex SNP genotyping. © 2009 American Chemical Society.