Multiple RONS-Loaded Plasma-Activated Ice Microneedle Patches for Transdermal Treatment of Psoriasis

Tong Wu; Jishen Zhang; Xixi Jing; Zewei Wang; Zijin Wu; Hao Zhang; Dingxin Liu; Mingzhe Rong; Paul K. Chu

doi:10.1021/acsami.4c10067

Multiple RONS-Loaded Plasma-Activated Ice Microneedle Patches for Transdermal Treatment of Psoriasis

Tong Wu, Jishen Zhang, Xixi Jing, Zewei Wang, Zijin Wu, Hao Zhang^*, Dingxin Liu^*, Mingzhe Rong, Paul K. Chu

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

14 Citations (Scopus)

Abstract

Cold atmospheric plasma (CAP) is a fledgling therapeutic technique for psoriasis treatment with noninvasiveness, but clinical adoption has been stifled by the insufficient production and delivery of plasma-generated reactive oxygen and nitrogen species (RONS). Herein, patches of air-discharge plasma-activated ice microneedles (PA-IMNs) loaded with multiple RONS are designed for local transdermal delivery to treat psoriasis as an alternative to direct CAP irradiation treatment. By mixing two RONS generated by the air-discharge plasma in the NO_x mode and O₃ mode, abundant high-valence RONS are produced and incorporated into PA-IMNs via complex gas−gas and gas−liquid reactions. The PA-IMNs abrogate keratinocyte overproliferation by inducing reactive oxygen species (ROS)-mediated loss of the mitochondrial membrane potential and apoptosis of keratinocytes. The in vivo transdermal treatment confirms that PA-IMNs produce significant anti-inflammatory and therapeutic actions for imiquimod (IMQ)-induced psoriasis-like dermatitis in mice by inhibiting the release of associated inflammatory factors while showing no evident systemic toxicity. Therefore, PA-IMNs have a large potential in transdermal delivery platforms as they overcome the limitations of using CAP directly in the clinical treatment of psoriasis. © 2024 American Chemical Society.

Original language	English
Pages (from-to)	46123-46132
Journal	ACS Applied Materials & Interfaces
Volume	16
Issue number	35
Online published	24 Aug 2024
DOIs	https://doi.org/10.1021/acsami.4c10067
Publication status	Published - 4 Sept 2024

Funding

This work was supported by the National Natural Science Foundation of China under Grant Nos. 52277231, 12175175, and 52307257, the City University of Hong Kong Donation Research Grants (DON-RMG 9229021 and 9220061), the Hong Kong PDFS─RGC Postdoctoral Fellowship Scheme (PDFS2122-1S08 and CityU 9061014), and the Hong Kong HMRF (Health and Medical Research Fund) (2120972 and CityU 9211320).

Research Keywords

cold atmospheric plasma
plasma-activated ice microneedles
psoriasis
reactive oxygen and nitrogen species
transdermal delivery

RGC Funding Information

RGC-funded

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10.1021/acsami.4c10067

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title = "Multiple RONS-Loaded Plasma-Activated Ice Microneedle Patches for Transdermal Treatment of Psoriasis",

abstract = "Cold atmospheric plasma (CAP) is a fledgling therapeutic technique for psoriasis treatment with noninvasiveness, but clinical adoption has been stifled by the insufficient production and delivery of plasma-generated reactive oxygen and nitrogen species (RONS). Herein, patches of air-discharge plasma-activated ice microneedles (PA-IMNs) loaded with multiple RONS are designed for local transdermal delivery to treat psoriasis as an alternative to direct CAP irradiation treatment. By mixing two RONS generated by the air-discharge plasma in the NOx mode and O3 mode, abundant high-valence RONS are produced and incorporated into PA-IMNs via complex gas−gas and gas−liquid reactions. The PA-IMNs abrogate keratinocyte overproliferation by inducing reactive oxygen species (ROS)-mediated loss of the mitochondrial membrane potential and apoptosis of keratinocytes. The in vivo transdermal treatment confirms that PA-IMNs produce significant anti-inflammatory and therapeutic actions for imiquimod (IMQ)-induced psoriasis-like dermatitis in mice by inhibiting the release of associated inflammatory factors while showing no evident systemic toxicity. Therefore, PA-IMNs have a large potential in transdermal delivery platforms as they overcome the limitations of using CAP directly in the clinical treatment of psoriasis. {\textcopyright} 2024 American Chemical Society.",

keywords = "cold atmospheric plasma, plasma-activated ice microneedles, psoriasis, reactive oxygen and nitrogen species, transdermal delivery",

author = "Tong Wu and Jishen Zhang and Xixi Jing and Zewei Wang and Zijin Wu and Hao Zhang and Dingxin Liu and Mingzhe Rong and Chu, {Paul K.}",

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TY - JOUR

T1 - Multiple RONS-Loaded Plasma-Activated Ice Microneedle Patches for Transdermal Treatment of Psoriasis

AU - Wu, Tong

AU - Zhang, Jishen

AU - Jing, Xixi

AU - Wang, Zewei

AU - Wu, Zijin

AU - Zhang, Hao

AU - Liu, Dingxin

AU - Rong, Mingzhe

AU - Chu, Paul K.

PY - 2024/9/4

Y1 - 2024/9/4

N2 - Cold atmospheric plasma (CAP) is a fledgling therapeutic technique for psoriasis treatment with noninvasiveness, but clinical adoption has been stifled by the insufficient production and delivery of plasma-generated reactive oxygen and nitrogen species (RONS). Herein, patches of air-discharge plasma-activated ice microneedles (PA-IMNs) loaded with multiple RONS are designed for local transdermal delivery to treat psoriasis as an alternative to direct CAP irradiation treatment. By mixing two RONS generated by the air-discharge plasma in the NOx mode and O3 mode, abundant high-valence RONS are produced and incorporated into PA-IMNs via complex gas−gas and gas−liquid reactions. The PA-IMNs abrogate keratinocyte overproliferation by inducing reactive oxygen species (ROS)-mediated loss of the mitochondrial membrane potential and apoptosis of keratinocytes. The in vivo transdermal treatment confirms that PA-IMNs produce significant anti-inflammatory and therapeutic actions for imiquimod (IMQ)-induced psoriasis-like dermatitis in mice by inhibiting the release of associated inflammatory factors while showing no evident systemic toxicity. Therefore, PA-IMNs have a large potential in transdermal delivery platforms as they overcome the limitations of using CAP directly in the clinical treatment of psoriasis. © 2024 American Chemical Society.

AB - Cold atmospheric plasma (CAP) is a fledgling therapeutic technique for psoriasis treatment with noninvasiveness, but clinical adoption has been stifled by the insufficient production and delivery of plasma-generated reactive oxygen and nitrogen species (RONS). Herein, patches of air-discharge plasma-activated ice microneedles (PA-IMNs) loaded with multiple RONS are designed for local transdermal delivery to treat psoriasis as an alternative to direct CAP irradiation treatment. By mixing two RONS generated by the air-discharge plasma in the NOx mode and O3 mode, abundant high-valence RONS are produced and incorporated into PA-IMNs via complex gas−gas and gas−liquid reactions. The PA-IMNs abrogate keratinocyte overproliferation by inducing reactive oxygen species (ROS)-mediated loss of the mitochondrial membrane potential and apoptosis of keratinocytes. The in vivo transdermal treatment confirms that PA-IMNs produce significant anti-inflammatory and therapeutic actions for imiquimod (IMQ)-induced psoriasis-like dermatitis in mice by inhibiting the release of associated inflammatory factors while showing no evident systemic toxicity. Therefore, PA-IMNs have a large potential in transdermal delivery platforms as they overcome the limitations of using CAP directly in the clinical treatment of psoriasis. © 2024 American Chemical Society.

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ER -

Multiple RONS-Loaded Plasma-Activated Ice Microneedle Patches for Transdermal Treatment of Psoriasis

Abstract

Funding

Research Keywords

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DON_RMG: Fabrication, Characterization, and Properties of Functional Materials - RMGS

DON: Surface Modification and Fabrication of Advanced Materials

HMRF: Biocompatible Titanium Implants with Antibacterial Capability Arising from the Synergistic Effects of Mechanical and Electrical Interactions and Quantitative Bacteria Sensing Ability Based on Electron Transfer

Cite this

Multiple RONS-Loaded Plasma-Activated Ice Microneedle Patches for Transdermal Treatment of Psoriasis

Abstract

Funding

Research Keywords

RGC Funding Information

Access Output

Other Access Options

Permanent Link

Other Files and Links

Fingerprint

Projects

DON_RMG: Fabrication, Characterization, and Properties of Functional Materials - RMGS

DON: Surface Modification and Fabrication of Advanced Materials

HMRF: Biocompatible Titanium Implants with Antibacterial Capability Arising from the Synergistic Effects of Mechanical and Electrical Interactions and Quantitative Bacteria Sensing Ability Based on Electron Transfer

Cite this