Multifunctional core-shell polymeric nanoparticles for transdermal DNA delivery and epidermal Langerhans cells tracking

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • Po-Wei Lee
  • Sheng-Hsiang Hsu
  • Jin-Sheng Tsai
  • Pei-Jai Huang
  • Cherng-Jyh Ke
  • Zi-Xian Liao
  • Chun-Wen Hsiao
  • Hao-Jan Lin
  • Hsing-Wen Sung

Detail(s)

Original languageEnglish
Pages (from-to)2425-2434
Journal / PublicationBiomaterials
Volume31
Issue number8
Online published19 Jan 2010
Publication statusPublished - Mar 2010
Externally publishedYes

Abstract

Skin is a highly immune-reactive tissue containing abundant antigen-presenting cells such as Langerhans cells (LCs), and thus is a favorable site for DNA immunization. This study developed a multifunctional core-shell nanoparticle system, which can be delivered transdermally into the epidermis via a gene gun, for use as a DNA carrier. The developed nanoparticles comprised a hydrophobic PLGA core and a positively-charged glycol chitosan (GC) shell. The core of the nanoparticles was used to load fluorescent quantum dots (QDs) for ultrasensitive detection of Langerhans cell migration following transdermal delivery, while a reporter gene was electrostatically adsorbed onto the GC shell layer of the nanoparticles. Results of fluorescence spectrophotometry, transmission electron microscopy, energy dispersive X-ray analysis, and X-ray diffraction measurement confirmed that the prepared nanoparticles had a core-shell structure with QDs in their core area. The surface charge of nanoparticles depended strongly on pH environment, enabling the intracellular release of the loaded DNA via a pH-mediated mechanism. Using a mouse model, this study demonstrated that bombardment of nanoparticles transfected DNA directly into LCs present in the epidermis; the transfected LCs then migrated and expressed the encoded gene products in the skin draining lymph nodes. These observation results suggest that the developed nanoparticle system is suitable for monitoring and fine-tuning important functional aspects of the immune system, in conjunction with the loaded fluorescence, and thus has potential for use in immunotherapy and vaccine development.

Research Area(s)

  • Cell tracking, Gene delivery, Immunotherapy, Quantum dots, Stimuli-responsive materials

Citation Format(s)

Multifunctional core-shell polymeric nanoparticles for transdermal DNA delivery and epidermal Langerhans cells tracking. / Lee, Po-Wei; Hsu, Sheng-Hsiang; Tsai, Jin-Sheng; Chen, Fu-Rong; Huang, Pei-Jai; Ke, Cherng-Jyh; Liao, Zi-Xian; Hsiao, Chun-Wen; Lin, Hao-Jan; Sung, Hsing-Wen.

In: Biomaterials, Vol. 31, No. 8, 03.2010, p. 2425-2434.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal