Multifunctional cationic polymer decorated and drug intercalated layered silicate (NLS) for early gastric cancer prevention

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

16 Scopus Citations
View graph of relations

Author(s)

  • Xue Jin
  • Xiurong Hu
  • Qiwen Wang
  • Kai Wang
  • Qi Yao
  • Guping Tang

Detail(s)

Original languageEnglish
Pages (from-to)3298-3308
Journal / PublicationBiomaterials
Volume35
Issue number10
Online published16 Jan 2014
Publication statusPublished - Mar 2014

Abstract

A multifunctional compound that can prevent early gastric cancer is produced by intercalating 3.20% and 1.64% of 5-FU into the interlayer of montmorillonite (MMT) and attapulgite (At), respectively. A low molecular weight cationic polymer, polyethylenimine (PEI1200), is incorporated into the surface of the 5-FU-MMT and 5-FU-At to form the multifunctional layered silicate (NLS). The chemical structure and surface morphology of the NLS are characterized and the model drug of 5-FU is intercalated into the MMT and At. The cell viability determined by the MTT assay on the BGC-823 cell lines show that over 80% of the cells are live under the experimental conditions. The PEI-5-FU-MMT and PEI-5-FU-At can carry the report gene to the BGC-823 and COS-7 cell lines efficiently. Western blotting assay shows that the pTrail protein of the BGC-823 cell lines treated with PEI-5-FU-MMT/pTrail and PEI-5-FU-At/pTrail is up-regulated, whereas the cFLIP protein is down-regulated at 48 h compared to free 5-FU, PEI1200, MMT, and At, providing evidence that the NLS can increase the sensitivity of pTrail gene and improve the effects of pTrail gene therapy. Moreover, the Helicobacter pylori (HP) bacteria are adsorbed and immobilized efficiently on the surface of the NLS according to the LIVE/DEAD® BacLight™ Bacterial Viability Kit in the confocal fluorescence analysis. The histochemical analyses provide evidence that NLS/pTrail can prevent early gastric mucosa effectively. © 2013 Elsevier Ltd.

Research Area(s)

  • Attapulgite, Cationic polymer, Drug intercalate, Gastric cancer, Montmorillonite

Citation Format(s)

Multifunctional cationic polymer decorated and drug intercalated layered silicate (NLS) for early gastric cancer prevention. / Jin, Xue; Hu, Xiurong; Wang, Qiwen; Wang, Kai; Yao, Qi; Tang, Guping; Chu, Paul K.

In: Biomaterials, Vol. 35, No. 10, 03.2014, p. 3298-3308.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review