More than one antibody of individual B cells revealed by single-cell immune profiling

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • Zhan Shi
  • Qingyang Zhang
  • Huige Yan
  • Ying Yang
  • Pingzhang Wang
  • Yixiao Zhang
  • Zhenling Deng
  • Meng Yu
  • Wenjing Zhou
  • Qianqian Wang
  • Xi Yang
  • Xiaoning Mo
  • Chi Zhang
  • Jing Huang
  • Hui Dai
  • Baofa Sun
  • Yongliang Zhao
  • Yun-Gui Yang
  • Xiaoyan Qiu

Detail(s)

Original languageEnglish
Article number64
Journal / PublicationCell Discovery
Volume5
Online published10 Dec 2019
Publication statusPublished - 2019

Abstract

Antibodies have a common structure consisting of two identical heavy (H) and two identical light (L) chains. It is widely accepted that a single mature B cell produces a single antibody through restricted synthesis of only one VHDJH (encoding the H-chain variable region) and one VLJL (encoding the L-chain variable region) via recombination. Naive B cells undergo class-switch recombination (CSR) from initially producing membrane-bound IgM and IgD to expressing more effective membrane-bound IgG, IgA, or IgE when encountering antigens. To ensure the “one cell — one antibody” paradigm, only the constant region of the H chain is replaced during CSR, while the rearranged VHDJH pattern and the L chain are kept unchanged. To define those long-standing classical concepts at the single-cell transcriptome level, we applied the Chromium Single-Cell Immune Profiling Solution and Sanger sequencing to evaluate the Ig transcriptome repertoires of single B cells. Consistent with the “one cell — one antibody” rule, most of the B cells showed one V(D)J recombination pattern. Intriguingly, however, two or more VHDJH or VLJL recombination patterns of IgH chain or IgL chain were also observed in hundreds to thousands of single B cells. Moreover, each Ig class showed unique VHDJH recombination pattern in a single B-cell expressing multiple Ig classes. Together, our findings reveal an unprecedented presence of multi-Ig specificity in some single B cells, implying regulation of Ig gene rearrangement and class switching that differs from the classical mechanisms of both the “one cell — one antibody” rule and CSR.

Research Area(s)

  • CLASS-SWITCH RECOMBINATION, V(D)J RECOMBINATION, ALLELIC EXCLUSION, CLONAL SELECTION, DNA DEAMINATION, CHAIN GENES, IMMUNOGLOBULIN, TRANSCRIPTION, ORGANIZATION, MECHANISMS

Citation Format(s)

More than one antibody of individual B cells revealed by single-cell immune profiling. / Shi, Zhan; Zhang, Qingyang; Yan, Huige; Yang, Ying; Wang, Pingzhang; Zhang, Yixiao; Deng, Zhenling; Yu, Meng; Zhou, Wenjing; Wang, Qianqian; Yang, Xi; Mo, Xiaoning; Zhang, Chi; Huang, Jing; Dai, Hui; Sun, Baofa; Zhao, Yongliang; Zhang, Liang; Yang, Yun-Gui; Qiu, Xiaoyan.

In: Cell Discovery, Vol. 5, 64, 2019.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal