Monodisperse Magnetite Nanoparticles Coupled with Nuclear Localization Signal Peptide for Cell-Nucleus Targeting

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

49 Scopus Citations
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Author(s)

  • Jin Xie
  • Nathan Kohler
  • Edward G. Walsh
  • Y. Eugene Chin
  • Shouheng Sun

Detail(s)

Original languageEnglish
Pages (from-to)548-552
Journal / PublicationChemistry - An Asian Journal
Volume3
Issue number3
Online published29 Feb 2008
Publication statusPublished - 7 Mar 2008
Externally publishedYes

Abstract

Functionalization of monodisperse superparamagnetic magnetite (Fe3O4) nanoparticles for cell specific targeting is crucial for cancer diagnostics and therapeutics. Targeted magnetic nanoparticles can be used to enhance the tissue contrast in magnetic resonance imaging (MRI), to improve the efficiency in anticancer drug delivery, and to eliminate tumor cells by magnetic fluid hyperthermia. Herein we report the nucleus-targeting Fe3O4 nanoparticles functionalized with protein and nuclear localization signal (NLS) peptide. These NLS-coated nanoparticles were introduced into the HeLa cell cytoplasm and nucleus, where the particles were monodispersed and non-aggregated. The success of labeling was examined and identified by fluorescence microscopy and MRI. The work demonstrates that monodisperse magnetic nanoparticles can be readily functionalized and stabilized for potential diagnostic and therapeutic applications. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

Research Area(s)

  • Cell imaging, Magnetic resonance imaging, Magnetite, Nanoparticles

Citation Format(s)

Monodisperse Magnetite Nanoparticles Coupled with Nuclear Localization Signal Peptide for Cell-Nucleus Targeting. / Xu, Chenjie; Xie, Jin; Kohler, Nathan; Walsh, Edward G.; Chin, Y. Eugene; Sun, Shouheng.

In: Chemistry - An Asian Journal, Vol. 3, No. 3, 07.03.2008, p. 548-552.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review