Molecular Tracking of Leishmania major in an Archived Rattus norvegicus Spleen Sample in Iran: A Case Report

Mahya Allahmoradi; Faezeh Najafi; Mona Koosha; Elham Kazemirad; Alireza Latifi; Saied Reza Naddaf; Hamed Mirjalali; Mehdi Mohebali; Homa Hajjaran; Domenico Otranto; Gholamreza Mowlavi

doi:10.18502/ijpa.v19i4.17172

Molecular Tracking of Leishmania major in an Archived Rattus norvegicus Spleen Sample in Iran: A Case Report

Mahya Allahmoradi, Faezeh Najafi, Mona Koosha, Elham Kazemirad, Alireza Latifi, Saied Reza Naddaf, Hamed Mirjalali, Mehdi Mohebali, Homa Hajjaran, Domenico Otranto, Gholamreza Mowlavi^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

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Abstract

Rodents are the primary reservoir hosts for zoonotic cutaneous leishmaniasis (ZCL) caused by Leishmania major. Knowing reservoir hosts is crucial for leishmaniasis sur-veillance and control programs in endemic areas. In this study, we examined an archived spleen of Rattus norvegicus obtained during a pest control program in 2000 in Tehran, the capital of Iran. The sample was analyzed using polymerase chain reaction (PCR) and sequencing to determine the presence of Trypanosomatidae based on the internal transcribed spacer (ITS) 1 gene. Amplification and sequencing of the dis-criminative region of the ITS1 gene followed by BLAST analysis showed the highest similarity with L. major isolates. Also, the phylogenetic analysis revealed that our sample was grouped with L. major isolates retrieved from the GenBank database. This finding might support the claim that R. norvegicus acts as a potential reservoir host for L. major. Further studies, including a survey on more rodent samples as well as stud-ying sandflies in the area, might uncover the possible presence of such pathobiolog-ical conditions in ZCL transmission in urban and suburban settings. © 2024 Allahmoradi et al.

Original language	English
Pages (from-to)	496-501
Journal	Iranian Journal of Parasitology
Volume	19
Issue number	4
DOIs	https://doi.org/10.18502/ijpa.v19i4.17172
Publication status	Published - Oct 2024
Externally published	Yes

Research Keywords

Iran
L. major
Rattus norvegicus
Rodents
Zoonotic cutaneous leishmaniasis

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This full text is made available under CC-BY-NC 4.0. https://creativecommons.org/licenses/by-nc/4.0/

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title = "Molecular Tracking of Leishmania major in an Archived Rattus norvegicus Spleen Sample in Iran: A Case Report",

abstract = "Rodents are the primary reservoir hosts for zoonotic cutaneous leishmaniasis (ZCL) caused by Leishmania major. Knowing reservoir hosts is crucial for leishmaniasis sur-veillance and control programs in endemic areas. In this study, we examined an archived spleen of Rattus norvegicus obtained during a pest control program in 2000 in Tehran, the capital of Iran. The sample was analyzed using polymerase chain reaction (PCR) and sequencing to determine the presence of Trypanosomatidae based on the internal transcribed spacer (ITS) 1 gene. Amplification and sequencing of the dis-criminative region of the ITS1 gene followed by BLAST analysis showed the highest similarity with L. major isolates. Also, the phylogenetic analysis revealed that our sample was grouped with L. major isolates retrieved from the GenBank database. This finding might support the claim that R. norvegicus acts as a potential reservoir host for L. major. Further studies, including a survey on more rodent samples as well as stud-ying sandflies in the area, might uncover the possible presence of such pathobiolog-ical conditions in ZCL transmission in urban and suburban settings. {\textcopyright} 2024 Allahmoradi et al.",

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doi = "10.18502/ijpa.v19i4.17172",

language = "English",

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publisher = "Teheran University of Medical Sciences",

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TY - JOUR

T1 - Molecular Tracking of Leishmania major in an Archived Rattus norvegicus Spleen Sample in Iran

T2 - A Case Report

AU - Allahmoradi, Mahya

AU - Najafi, Faezeh

AU - Koosha, Mona

AU - Kazemirad, Elham

AU - Latifi, Alireza

AU - Naddaf, Saied Reza

AU - Mirjalali, Hamed

AU - Mohebali, Mehdi

AU - Hajjaran, Homa

AU - Otranto, Domenico

AU - Mowlavi, Gholamreza

PY - 2024/10

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N2 - Rodents are the primary reservoir hosts for zoonotic cutaneous leishmaniasis (ZCL) caused by Leishmania major. Knowing reservoir hosts is crucial for leishmaniasis sur-veillance and control programs in endemic areas. In this study, we examined an archived spleen of Rattus norvegicus obtained during a pest control program in 2000 in Tehran, the capital of Iran. The sample was analyzed using polymerase chain reaction (PCR) and sequencing to determine the presence of Trypanosomatidae based on the internal transcribed spacer (ITS) 1 gene. Amplification and sequencing of the dis-criminative region of the ITS1 gene followed by BLAST analysis showed the highest similarity with L. major isolates. Also, the phylogenetic analysis revealed that our sample was grouped with L. major isolates retrieved from the GenBank database. This finding might support the claim that R. norvegicus acts as a potential reservoir host for L. major. Further studies, including a survey on more rodent samples as well as stud-ying sandflies in the area, might uncover the possible presence of such pathobiolog-ical conditions in ZCL transmission in urban and suburban settings. © 2024 Allahmoradi et al.

AB - Rodents are the primary reservoir hosts for zoonotic cutaneous leishmaniasis (ZCL) caused by Leishmania major. Knowing reservoir hosts is crucial for leishmaniasis sur-veillance and control programs in endemic areas. In this study, we examined an archived spleen of Rattus norvegicus obtained during a pest control program in 2000 in Tehran, the capital of Iran. The sample was analyzed using polymerase chain reaction (PCR) and sequencing to determine the presence of Trypanosomatidae based on the internal transcribed spacer (ITS) 1 gene. Amplification and sequencing of the dis-criminative region of the ITS1 gene followed by BLAST analysis showed the highest similarity with L. major isolates. Also, the phylogenetic analysis revealed that our sample was grouped with L. major isolates retrieved from the GenBank database. This finding might support the claim that R. norvegicus acts as a potential reservoir host for L. major. Further studies, including a survey on more rodent samples as well as stud-ying sandflies in the area, might uncover the possible presence of such pathobiolog-ical conditions in ZCL transmission in urban and suburban settings. © 2024 Allahmoradi et al.

KW - Iran

KW - L. major

KW - Rattus norvegicus

KW - Rodents

KW - Zoonotic cutaneous leishmaniasis

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JO - Iranian Journal of Parasitology

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ER -

Molecular Tracking of Leishmania major in an Archived Rattus norvegicus Spleen Sample in Iran: A Case Report

Abstract

Research Keywords

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