TY - JOUR
T1 - Molecular staging of marine medaka
T2 - A model organism for marine ecotoxicity study
AU - Chen, Xueping
AU - Li, Li
AU - Cheng, Jinping
AU - Chan, Leo Lai
AU - Wang, Da-Zhi
AU - Wang, Ke-Jian
AU - Baker, Michael E.
AU - Hardiman, Gary
AU - Schlenk, Daniel
AU - Cheng, Shuk Han
PY - 2011
Y1 - 2011
N2 - Oryzias melastigma, also called O. dancena, is becoming a very useful model for estuarine and marine ecotoxicity studies. With O. melastigma being adopted by ILSI Health and Environmental Science Institute (HESI) for embryo toxicity testing, improved knowledge of biomarker based embryonic development becomes especially important for mechanism-based toxicity evaluations. Using whole mount in situ hybridization and immunostaining techniques together with widely used molecular markers, this study describes the molecular development of marine medaka embryos, focusing on the brain, eye, heart, pectoral fin, pancreas, liver, muscle and neuron system. These organs are targets of environmental pollutants that disrupt normal embryonic development in medaka and other fish. © 2011 Elsevier Ltd.
AB - Oryzias melastigma, also called O. dancena, is becoming a very useful model for estuarine and marine ecotoxicity studies. With O. melastigma being adopted by ILSI Health and Environmental Science Institute (HESI) for embryo toxicity testing, improved knowledge of biomarker based embryonic development becomes especially important for mechanism-based toxicity evaluations. Using whole mount in situ hybridization and immunostaining techniques together with widely used molecular markers, this study describes the molecular development of marine medaka embryos, focusing on the brain, eye, heart, pectoral fin, pancreas, liver, muscle and neuron system. These organs are targets of environmental pollutants that disrupt normal embryonic development in medaka and other fish. © 2011 Elsevier Ltd.
KW - Immunostaining
KW - In situ hybridization
KW - Medaka
KW - Molecular staging
KW - Oryzias melastigma
UR - http://www.scopus.com/inward/record.url?scp=79960357244&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-79960357244&origin=recordpage
U2 - 10.1016/j.marpolbul.2011.03.042
DO - 10.1016/j.marpolbul.2011.03.042
M3 - RGC 21 - Publication in refereed journal
C2 - 21708389
SN - 0025-326X
VL - 63
SP - 309
EP - 317
JO - Marine Pollution Bulletin
JF - Marine Pollution Bulletin
IS - 5-12
ER -
