Molecular spectra of acrylamide-induced mutation at hprt locus in human promyelocytic leukemia HL-60 and NB4 cell lines

The genotoxicity of acrylamide was investigated by methods of single cell clone culturing, two-way screening count, multiplex PCR amplification and electrophoresis technique. Acrylamide only showed clear mutagenesis until dose 700 mg · L^-1 in HL-60 cells. The most frequent spontaneous mutation was point mutation(≥90.0%) and acrylamide-induced mutation mainly included partial deletion and point mutation (respectively 40.0% - 66.7%, 33.3% - 60.0%). Total gene deletion was not discovered in both of cells. There was deletion mutation in all exons of hprt gene(except 7/8 exon), and toward the 3′ end of the hprt gene. The most frequent acrylamide-induced mutations were point mutation and single exon deletion (93.3 %, 86.1%). There was no clear difference in both of cells. The results suggest that the spectra of spontaneous and acrylamide-induced mutants are different, and the smaller changes in genetic structure have something to do with mechanism.