Molecular detection and risk factors associated with multidrug-resistant Campylobacter jejuni from broiler cloacal and meat samples in Bangladesh

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Md. Sirazul Islam
  • Chandan Nath
  • Jahan Ara
  • Tahia Ahmed Logno
  • Md. Helal Uddin
  • Md. Ibrahim Khalil
  • Pronesh Dutta
  • Tridip Das
  • Sharmin Chowdhury

Detail(s)

Original languageEnglish
Journal / PublicationZoonoses and Public Health
Online published26 May 2022
Publication statusOnline published - 26 May 2022

Abstract

The gastrointestinal tract of poultry is a potential source of Campylobacter jejuni. Here, the prevalence, risk factors, antimicrobial susceptibility profile and genetic relationship of C. jejuni were studied in broilers from farms and meat from live bird markets (LBMs) and super shops (SS). Pooled cloacal samples were obtained from farms in six districts of Bangladesh between June 2019 and March 2020. Pooled meat samples were obtained from LBMs and SS in the Chattogram district. Microbial culture, polymerase chain reaction (PCR), antimicrobial susceptibility tests were used to detect multidrug-resistant C. jejuni. A positive PCR amplicon was validated by mapA partial gene sequencing and subsequent phylogenetic analysis. In total, 12.5% (95% CI: 8.5–17.7%) of farms (N = 216) and 27.1% (95% CI: 15.28–41.85%) of LBMs and SS (= 48) tested positive for C. jejuni. Moreover, 98% of the isolates were multidrug-resistant, with 86% resistant to five or more antimicrobial groups. Multivariable logistic regression analysis showed a downtime of <14 days, no separate footwear for shed access, and more than one person entering the sheds were significantly associated with C. jejuni colonization. Phylogenetic analysis revealed a strong relationship between C. jejuni strains obtained in Bangladesh and strains isolated in India, South Africa and Grenada from humans, pigs and bats. This study revealed significant contamination of broiler meat with Campylobacter spp. and C. jejuni. Potential sources of contamination and anthropogenic factors associated with the alarming prevalence of C. jejuni identified in this study would aid in reducing the growing risks of broiler-associated pathogens.

Research Area(s)

  • Campylobacter spp., multidrug resistance, prevalence, public health, zoonoses

Citation Format(s)

Molecular detection and risk factors associated with multidrug-resistant Campylobacter jejuni from broiler cloacal and meat samples in Bangladesh. / Islam, Md. Sirazul; Hasib, Farazi Muhammad Yasir; Nath, Chandan; Ara, Jahan; Logno, Tahia Ahmed; Uddin, Md. Helal; Khalil, Md. Ibrahim; Dutta, Pronesh; Das, Tridip; Chowdhury, Sharmin.

In: Zoonoses and Public Health, 26.05.2022.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review