Molecular cloning and characterization of a hypoxia-responsive CITED3 cDNA from grass carp

P. K S Ng; R. S S Wu; Z. P. Zhang; H. O L Mok; D. J. Randall; R. Y C Kong

doi:10.1016/S1096-4959(03)00224-0

Molecular cloning and characterization of a hypoxia-responsive CITED3 cDNA from grass carp

P. K S Ng, R. S S Wu, Z. P. Zhang, H. O L Mok, D. J. Randall, R. Y C Kong

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

7 Citations (Scopus)

Abstract

We have isolated a 1586-bp full-length CITED3 cDNA from grass carp which specifies for a cAMP-responsive element-binding protein/p300-interacting transactivator with glutamic acid (E)/aspartic acid (D)-rich C-terminal domain protein. The cDNA, designated as gcCITED3, has an open reading frame of 762 bp and encodes a protein of 253 amino acids with a predicted molecular mass of 28. 3 kDa and pI of 6.4. Pairwise comparison showed that gcCITED3 shares high sequence identity with the CITED3 of zebrafish (94%), chicken (72%) and Xenopus (59%). Northern blot analysis indicated that gcCITED3 is most highly expressed and responsive to hypoxia in the carp kidney. Hypoxic induction was also observed in heart, albeit at a lower level. This is the first report on the isolation of a hypoxia-responsive CITED3 gene from fish. © 2003 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	163-172
Journal	Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume	136
Issue number	2
DOIs	https://doi.org/10.1016/S1096-4959(03)00224-0
Publication status	Published - Oct 2003

Research Keywords

CITED
Co-transactivator
Ctenopharyngodon idellus
Gene regulation
Grass carp
Hypoxia
RACE PCR

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title = "Molecular cloning and characterization of a hypoxia-responsive CITED3 cDNA from grass carp",

abstract = "We have isolated a 1586-bp full-length CITED3 cDNA from grass carp which specifies for a cAMP-responsive element-binding protein/p300-interacting transactivator with glutamic acid (E)/aspartic acid (D)-rich C-terminal domain protein. The cDNA, designated as gcCITED3, has an open reading frame of 762 bp and encodes a protein of 253 amino acids with a predicted molecular mass of 28. 3 kDa and pI of 6.4. Pairwise comparison showed that gcCITED3 shares high sequence identity with the CITED3 of zebrafish (94%), chicken (72%) and Xenopus (59%). Northern blot analysis indicated that gcCITED3 is most highly expressed and responsive to hypoxia in the carp kidney. Hypoxic induction was also observed in heart, albeit at a lower level. This is the first report on the isolation of a hypoxia-responsive CITED3 gene from fish. {\textcopyright} 2003 Elsevier Inc. All rights reserved.",

keywords = "CITED, Co-transactivator, Ctenopharyngodon idellus, Gene regulation, Grass carp, Hypoxia, RACE PCR",

author = "Ng, {P. K S} and Wu, {R. S S} and Zhang, {Z. P.} and Mok, {H. O L} and Randall, {D. J.} and Kong, {R. Y C}",

year = "2003",

month = oct,

doi = "10.1016/S1096-4959(03)00224-0",

language = "English",

volume = "136",

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journal = "Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology",

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TY - JOUR

T1 - Molecular cloning and characterization of a hypoxia-responsive CITED3 cDNA from grass carp

AU - Ng, P. K S

AU - Wu, R. S S

AU - Zhang, Z. P.

AU - Mok, H. O L

AU - Randall, D. J.

AU - Kong, R. Y C

PY - 2003/10

Y1 - 2003/10

N2 - We have isolated a 1586-bp full-length CITED3 cDNA from grass carp which specifies for a cAMP-responsive element-binding protein/p300-interacting transactivator with glutamic acid (E)/aspartic acid (D)-rich C-terminal domain protein. The cDNA, designated as gcCITED3, has an open reading frame of 762 bp and encodes a protein of 253 amino acids with a predicted molecular mass of 28. 3 kDa and pI of 6.4. Pairwise comparison showed that gcCITED3 shares high sequence identity with the CITED3 of zebrafish (94%), chicken (72%) and Xenopus (59%). Northern blot analysis indicated that gcCITED3 is most highly expressed and responsive to hypoxia in the carp kidney. Hypoxic induction was also observed in heart, albeit at a lower level. This is the first report on the isolation of a hypoxia-responsive CITED3 gene from fish. © 2003 Elsevier Inc. All rights reserved.

AB - We have isolated a 1586-bp full-length CITED3 cDNA from grass carp which specifies for a cAMP-responsive element-binding protein/p300-interacting transactivator with glutamic acid (E)/aspartic acid (D)-rich C-terminal domain protein. The cDNA, designated as gcCITED3, has an open reading frame of 762 bp and encodes a protein of 253 amino acids with a predicted molecular mass of 28. 3 kDa and pI of 6.4. Pairwise comparison showed that gcCITED3 shares high sequence identity with the CITED3 of zebrafish (94%), chicken (72%) and Xenopus (59%). Northern blot analysis indicated that gcCITED3 is most highly expressed and responsive to hypoxia in the carp kidney. Hypoxic induction was also observed in heart, albeit at a lower level. This is the first report on the isolation of a hypoxia-responsive CITED3 gene from fish. © 2003 Elsevier Inc. All rights reserved.

KW - CITED

KW - Co-transactivator

KW - Ctenopharyngodon idellus

KW - Gene regulation

KW - Grass carp

KW - Hypoxia

KW - RACE PCR

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DO - 10.1016/S1096-4959(03)00224-0

M3 - RGC 21 - Publication in refereed journal

C2 - 14529742

SN - 1096-4959

VL - 136

SP - 163

EP - 172

JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

IS - 2

ER -

Molecular cloning and characterization of a hypoxia-responsive CITED3 cDNA from grass carp

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