Molecular characterization of potentially zoonotic isolates of Giardia duodenalis in horses

Rebecca Traub, Susan Wade, Carolyn Read, Andrew Thompson, Hussni Mohammed

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

44 Citations (Scopus)

Abstract

Giardia isolates from eight horses from New York State (NY), USA and two horses from Western Australia (WA) were genetically characterized at the SSU-rDNA and triose-phosphate isomerase (TPI) genes. Phylogenetic analysis of the TPI gene provided strong support for the placement of both isolates of Giardia from horses in WA and a single isolate from a horse in NY within the assemblage AI genotype of G. duodenalis. Another two isolates from horses in NY placed within the assemblage AII genotype of G. duodenalis. Phylogenetic analysis of the TPI gene also provided strong bootstrap support for the placement of four G. duodenalis isolates from horses in NY into a potentially host-specific sub-assemblage of assemblage BIV. The results of this study are consistent with previous studies showing that assemblages AI and AII of G. duodenalis provide the greatest potential zoonotic risk to humans. Horses may therefore constitute a potential source for human infection of Giardia either directly or via watersheds. © 2005 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)317-321
JournalVeterinary Parasitology
Volume130
Issue number3-4
DOIs
Publication statusPublished - 30 Jun 2005
Externally publishedYes

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Research Keywords

  • Giardia
  • Horses
  • PCR
  • Ribosomal
  • Triose phosphate isomerase

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