Molecular characterization of Eimeria spp. from chicken by Polymerase Chain Reaction based on species-specific SCAR markers

AMAM Zonaed Siddiki; Sohana Akter Mina; Md. Anayet Hasan; Mohammed Touaha Akbar; Rashel Alam; Md. Ashraful Islam; Israt Zahan Ira; Bibi Ayesa

doi:10.9790/2380-07111317

Molecular characterization of Eimeria spp. from chicken by Polymerase Chain Reaction based on species-specific SCAR markers

AMAM Zonaed Siddiki, Sohana Akter Mina, Md. Anayet Hasan, Mohammed Touaha Akbar, Rashel Alam, Md. Ashraful Islam, Israt Zahan Ira, Bibi Ayesa

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

Abstract

Coccidiosis in chickens is one of the major problems of poultry industry that is caused by protozoan parasites of genus Eimeria . Present study was conducted to characterize the Eimeria species infecting poultry in different regions of Chittagong district of Bangladesh. Seven species of Eimeria including E. tenella, E. necatrix, E. acervulina, E. maxima, E. brunetti, E. mitis, and E. praecox have been characterized using conventional PCR analysis through amplifying unique single copy sequences derived from sequence characterized amplified region (SCAR) markers. The present study is the first of its type to use molecular tools to identify Eimeria spp. infecting chicken in Bangladesh. The study indicates that the modern molecular technique involving PCR to detect the Eimeria species are more reliable and accurate than the traditional morphology-based technique.

Original language	English
Pages (from-to)	13-17
Journal	IOSR Journal of Agriculture and Veterinary Science
Volume	7
Issue number	Issue 1 Ver. I
DOIs	https://doi.org/10.9790/2380-07111317
Publication status	Published - Jan 2014
Externally published	Yes

Research Keywords

Eimeria
PCR
SCAR
Coccidiosis
Bangladesh

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10.9790/2380-07111317

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@article{323d7af0791b455586375381665f2022,

title = "Molecular characterization of Eimeria spp. from chicken by Polymerase Chain Reaction based on species-specific SCAR markers",

abstract = "Coccidiosis in chickens is one of the major problems of poultry industry that is caused by protozoan parasites of genus Eimeria . Present study was conducted to characterize the Eimeria species infecting poultry in different regions of Chittagong district of Bangladesh. Seven species of Eimeria including E. tenella, E. necatrix, E. acervulina, E. maxima, E. brunetti, E. mitis, and E. praecox have been characterized using conventional PCR analysis through amplifying unique single copy sequences derived from sequence characterized amplified region (SCAR) markers. The present study is the first of its type to use molecular tools to identify Eimeria spp. infecting chicken in Bangladesh. The study indicates that the modern molecular technique involving PCR to detect the Eimeria species are more reliable and accurate than the traditional morphology-based technique.",

keywords = "Eimeria, PCR, SCAR, Coccidiosis, Bangladesh",

author = "Siddiki, {AMAM Zonaed} and Mina, {Sohana Akter} and Hasan, {Md. Anayet} and Akbar, {Mohammed Touaha} and Rashel Alam and Islam, {Md. Ashraful} and Ira, {Israt Zahan} and Bibi Ayesa",

year = "2014",

month = jan,

doi = "10.9790/2380-07111317",

language = "English",

volume = "7",

pages = "13--17",

number = "Issue 1 Ver. I",

}

Molecular characterization of Eimeria spp. from chicken by Polymerase Chain Reaction based on species-specific SCAR markers. / Siddiki, AMAM Zonaed; Mina, Sohana Akter; Hasan, Md. Anayet et al.
In: IOSR Journal of Agriculture and Veterinary Science, Vol. 7, No. Issue 1 Ver. I, 01.2014, p. 13-17.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

TY - JOUR

T1 - Molecular characterization of Eimeria spp. from chicken by Polymerase Chain Reaction based on species-specific SCAR markers

AU - Siddiki, AMAM Zonaed

AU - Mina, Sohana Akter

AU - Hasan, Md. Anayet

AU - Akbar, Mohammed Touaha

AU - Alam, Rashel

AU - Islam, Md. Ashraful

AU - Ira, Israt Zahan

AU - Ayesa, Bibi

PY - 2014/1

Y1 - 2014/1

N2 - Coccidiosis in chickens is one of the major problems of poultry industry that is caused by protozoan parasites of genus Eimeria . Present study was conducted to characterize the Eimeria species infecting poultry in different regions of Chittagong district of Bangladesh. Seven species of Eimeria including E. tenella, E. necatrix, E. acervulina, E. maxima, E. brunetti, E. mitis, and E. praecox have been characterized using conventional PCR analysis through amplifying unique single copy sequences derived from sequence characterized amplified region (SCAR) markers. The present study is the first of its type to use molecular tools to identify Eimeria spp. infecting chicken in Bangladesh. The study indicates that the modern molecular technique involving PCR to detect the Eimeria species are more reliable and accurate than the traditional morphology-based technique.

AB - Coccidiosis in chickens is one of the major problems of poultry industry that is caused by protozoan parasites of genus Eimeria . Present study was conducted to characterize the Eimeria species infecting poultry in different regions of Chittagong district of Bangladesh. Seven species of Eimeria including E. tenella, E. necatrix, E. acervulina, E. maxima, E. brunetti, E. mitis, and E. praecox have been characterized using conventional PCR analysis through amplifying unique single copy sequences derived from sequence characterized amplified region (SCAR) markers. The present study is the first of its type to use molecular tools to identify Eimeria spp. infecting chicken in Bangladesh. The study indicates that the modern molecular technique involving PCR to detect the Eimeria species are more reliable and accurate than the traditional morphology-based technique.

KW - Eimeria

KW - PCR

KW - SCAR

KW - Coccidiosis

KW - Bangladesh

U2 - 10.9790/2380-07111317

DO - 10.9790/2380-07111317

M3 - RGC 21 - Publication in refereed journal

SN - 2319-2372

VL - 7

SP - 13

EP - 17

JO - IOSR Journal of Agriculture and Veterinary Science

JF - IOSR Journal of Agriculture and Veterinary Science

IS - Issue 1 Ver. I

ER -

Molecular characterization of Eimeria spp. from chicken by Polymerase Chain Reaction based on species-specific SCAR markers

Abstract

Research Keywords

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