Molecular Characterization of Escherichia coli Strains Isolated from Retail Meat That Harbor blaCTX-M and fosA3 Genes

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

16 Scopus Citations
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Author(s)

  • Dachuan Lin
  • Kaichao Chen
  • Edward Wai Chi Chan
  • Wen Yao

Detail(s)

Original languageEnglish
Pages (from-to)2450-2455
Journal / PublicationAntimicrobial Agents and Chemotherapy
Volume60
Issue number4
Online published25 Mar 2016
Publication statusPublished - Apr 2016
Externally publishedYes

Abstract

A total of 55 cefotaxime-resistant Escherichia coli isolates were obtained from retail meat products purchased in Shenzhen, China, during the period November 2012 to May 2013. Thirty-seven of these 55 isolates were found to harbor a blaCTX-M gene, with the blaCTX-M-1 group being the most common type. blaCMY-2 was detected in 16 isolates, alone or in combination with other extended-spectrum β-lactamase (ESBL) determinants. Importantly, the fosA3 gene, which encodes fosfomycin resistance, was detected in 12 isolates, with several being found to reside in the conjugative plasmid that harbored the blaCTX-M gene. The insertion sequence IS26 was observed upstream of some of the blaCTX-M-55 and fosA3 genes. Conjugation experiments showed that blaCTX-M genes from 15 isolates were transferrable, with Inc I1 and Inc FII being the most prevalent replicons. High clonal diversity was observed among the blaCTX-M producers, suggesting that horizontal transfer of the blaCTX-M genes among E. coli strains in retail meats is a common event and that such strains may constitute an important reservoir of blaCTX-M genes, which may be readily disseminated to other potential human pathogens.

Citation Format(s)

Molecular Characterization of Escherichia coli Strains Isolated from Retail Meat That Harbor blaCTX-M and fosA3 Genes. / Xie, Miaomiao; Lin, Dachuan; Chen, Kaichao; Chan, Edward Wai Chi; Yao, Wen; Chen, Sheng.

In: Antimicrobial Agents and Chemotherapy, Vol. 60, No. 4, 04.2016, p. 2450-2455.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review