Molecular Basis for Stellatic Acid Biosynthesis : A Genome Mining Approach for Discovery of Sesterterpene Synthases

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Original languageEnglish
Pages (from-to)4644-4647
Journal / PublicationOrganic Letters
Volume17
Issue number18
Online published9 Sep 2015
Publication statusPublished - 18 Sep 2015
Externally publishedYes

Abstract

The search for a new sesterterpene synthase in the genome of Emericella variecolor, which reportedly produces diverse sesterterpenoids, is described. One gene product (a chimeric protein with prenyltransferase and terpene cyclase domains) led to the synthesis of a novel tricyclic sesterterpene, stellata-2,6,19-triene (1), from DMAPP and IPP, and the hydrocarbon was further transformed into stellatic acid (2) by cytochrome P450 monooxygenase encoded by the gene adjacent to the sesterterpene synthase gene.