MOF-Derived Cu-BTC Nanowire-Embedded 2D Leaf-like Structured ZIF Composite-Based Aptamer Sensors for Real-Time In Vivo Insulin Monitoring

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Rajalakshmi Sakthivel
  • Lu-Yin Lin
  • Yeh-Fang Duann
  • Hsiao-Hsuan Chen
  • Chaochin Su
  • Xinke Liu
  • Ren-Jei Chung

Detail(s)

Original languageEnglish
Pages (from-to)28639-28650
Journal / PublicationACS Applied Materials & Interfaces
Volume14
Issue number25
Online published16 Jun 2022
Publication statusPublished - 29 Jun 2022

Abstract

Insulin, which is a hormone produced by the β-cells of the pancreas, regulates the glucose levels in the blood and can transport glucose into cells to produce glycogen or triglycerides. Insulin deficiency can lead to hyperglycemia and diabetes. Therefore, insulin detection is critical in clinical diagnosis. In this study, disposable Au electrodes were modified with copper(II) benzene-1,3,5-tricarboxylate (Cu-BTC)/leaf-like zeolitic imidazolate framework (ZIF-L) for insulin detection. The aptamers are easily immobilized on the Cu-BTC/ZIF-L composite by physical adsorption and facilitated the specific interaction between aptamers and insulin. The Cu-BTC/ZIF-L composite-based aptasensor presented a wide linear insulin detection range (0.1 pM to 5 μM) and a low limit of detection of 0.027 pM. In addition, the aptasensor displayed high specificity, good reproducibility and stability, and favorable practicability in human serum samples. For the in vivo tests, Cu-BTC/ZIF-L composite-modified electrodes were implanted in non-diabetic and diabetic mice, and insulin was quantified using electrochemical and enzyme-linked immunosorbent assay methods.

Research Area(s)

  • 3,5-tricarboxylate, aptamer, copper(II) benzene-1, disposable Au electrode, in vivo monitoring, insulin, leaf-like zeolitic imidazolate framework

Citation Format(s)

MOF-Derived Cu-BTC Nanowire-Embedded 2D Leaf-like Structured ZIF Composite-Based Aptamer Sensors for Real-Time In Vivo Insulin Monitoring. / Sakthivel, Rajalakshmi; Lin, Lu-Yin; Duann, Yeh-Fang; Chen, Hsiao-Hsuan; Su, Chaochin; Liu, Xinke; He, Jr-Hau; Chung, Ren-Jei.

In: ACS Applied Materials & Interfaces, Vol. 14, No. 25, 29.06.2022, p. 28639-28650.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review