Modulation of the mechanosensing of mesenchymal stem cells by laser-induced patterning for the acceleration of tissue reconstruction through the Wnt/β-catenin signaling pathway activation

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review

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Author(s)

  • Jieni Fu
  • Xiangmei Liu
  • Lei Tan
  • Zhenduo Cui
  • Yanqin Liang
  • Zhaoyang Li
  • Shengli Zhu
  • Yufeng Zheng
  • Kelvin Wai Kwok Yeung
  • Shuilin Wu

Detail(s)

Original languageEnglish
Pages (from-to)152-167
Journal / PublicationActa Biomaterialia
Volume101
Online published1 Nov 2019
Publication statusPublished - 1 Jan 2020

Abstract

Growing evidence suggests that the physical microenvironment can guide cell fate. However, cells sense cues from the adjacent physical microenvironment over a limited distance. In the present study, murine mesenchymal stem cells (MSCs) and murine preosteoblastic cells (MC3T3-E1) behaviors are regulated by the cell–material interface using ordered-micro and disordered-nano patterned structures on Ti implants. The optimal bone formation structure is a stable wave (horizontal direction: ridge, 2.7 µm; grooves, 5.3 µm; and vertical direction: distance, 700 µm) with the appropriate density of nano-branches (6.0 per µm2). The repeated waves provide cells with directional guidance, and the disordered branches influence cell geometry by providing different spacing and density nanostructure. And micro-nano patterned structure can provide biophysical cues to direct cell phenotype development, including cell size, shape, and orientation, to influence cellular processes including survival, growth, and differentiation. Thus, the overlaid isotropic and anisotropic cues, ordered-micro and disordered-nano patterned structures, could transfer further and alter cell shape and induce nuclear orientation by activating Wnt/β-catenin signaling to promote integrin α5integrin β1, cadherin 2, Runx2, Opn, and Ocn. That canonical Wnt signaling inhibitor dickkopf1 further demonstrates osteogenic differentiation induced by ordered-micro and disordered-nano patterned structures, which is related to Wnt/β-catenin signaling. Our findings show the role of ordered microstructures and disordered nanostructures in modulating stem cell differentiation with potential medical applications.

Research Area(s)

  • Implants, Laser, Mechanosensing, Mesenchymal stem cells, Signaling pathway, Surface modification

Citation Format(s)

Modulation of the mechanosensing of mesenchymal stem cells by laser-induced patterning for the acceleration of tissue reconstruction through the Wnt/β-catenin signaling pathway activation. / Fu, Jieni; Liu, Xiangmei; Tan, Lei; Cui, Zhenduo; Liang, Yanqin; Li, Zhaoyang; Zhu, Shengli; Zheng, Yufeng; Kwok Yeung, Kelvin Wai; Chu, Paul K; Wu, Shuilin.

In: Acta Biomaterialia, Vol. 101, 01.01.2020, p. 152-167.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review