Modified nucleic acid aptamers: development, characterization, and biological applications

Danyang Ji; Hengxin Feng; Shiau Wei Liew; Chun Kit Kwok

doi:10.1016/j.tibtech.2023.05.005

Modified nucleic acid aptamers: development, characterization, and biological applications

Danyang Ji (Co-first Author)
, Hengxin Feng (Co-first Author)
, Shiau Wei Liew (Co-first Author)
, Chun Kit Kwok^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

59 Citations (Scopus)

95 Downloads (CityUHK Scholars)

Abstract

Aptamers are single-stranded oligonucleotides that bind to their targets via specific structural interactions. To improve the properties and performance of aptamers, modified nucleotides are incorporated during or after a selection process such as systematic evolution of ligands by exponential enrichment (SELEX). We summarize the latest modified nucleotides and strategies used in modified (mod)-SELEX and post-SELEX to develop modified aptamers, highlight the methods used to characterize aptamer–target interactions, and present recent progress in modified aptamers that recognize different targets. We discuss the challenges and perspectives in further advancing the methodologies and toolsets to accelerate the discovery of modified aptamers, improve the throughput of aptamer-target characterization, and expand the functional diversity and complexity of modified aptamers. © 2023 Elsevier Ltd.

Original language	English
Pages (from-to)	1360-1384
Number of pages	25
Journal	Trends in Biotechnology
Volume	41
Issue number	11
Online published	10 Jun 2023
DOIs	https://doi.org/10.1016/j.tibtech.2023.05.005
Publication status	Published - Nov 2023

Funding

This work was supported by the Shenzhen Basic Research Project 487 [JCYJ20180507181642811]; NSFC Excellent Young Scientists Fund (Hong Kong and Macau) 488 Project [32222089]; Research Grants Council of the Hong Kong SAR, China Projects [CityU 489 11100222, CityU 11100421, CityU 11101519]; Croucher Foundation Project [9509003]; State 490 Key Laboratory of Marine Pollution Director Discretionary Fund [DDF/0008] and Seed 491 Collaborative Research Fund [SCRF/0037, SCRF/0040]; City University of Hong Kong 492 projects [9678302], [9667222] to C.K.K.. Hong Kong PhD Fellowship Scheme to S.W.L.

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COPYRIGHT TERMS OF DEPOSITED POSTPRINT FILE: © 2023. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/.

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title = "Modified nucleic acid aptamers: development, characterization, and biological applications",

abstract = "Aptamers are single-stranded oligonucleotides that bind to their targets via specific structural interactions. To improve the properties and performance of aptamers, modified nucleotides are incorporated during or after a selection process such as systematic evolution of ligands by exponential enrichment (SELEX). We summarize the latest modified nucleotides and strategies used in modified (mod)-SELEX and post-SELEX to develop modified aptamers, highlight the methods used to characterize aptamer–target interactions, and present recent progress in modified aptamers that recognize different targets. We discuss the challenges and perspectives in further advancing the methodologies and toolsets to accelerate the discovery of modified aptamers, improve the throughput of aptamer-target characterization, and expand the functional diversity and complexity of modified aptamers. {\textcopyright} 2023 Elsevier Ltd.",

author = "Danyang Ji and Hengxin Feng and Liew, \{Shiau Wei\} and Kwok, \{Chun Kit\}",

year = "2023",

month = nov,

doi = "10.1016/j.tibtech.2023.05.005",

language = "English",

volume = "41",

pages = "1360--1384",

journal = "Trends in Biotechnology",

issn = "0167-7799",

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Modified nucleic acid aptamers: development, characterization, and biological applications. / Ji, Danyang (Co-first Author); Feng, Hengxin (Co-first Author); Liew, Shiau Wei (Co-first Author) et al.
In: Trends in Biotechnology, Vol. 41, No. 11, 11.2023, p. 1360-1384.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

TY - JOUR

T1 - Modified nucleic acid aptamers

T2 - development, characterization, and biological applications

AU - Ji, Danyang

AU - Feng, Hengxin

AU - Liew, Shiau Wei

AU - Kwok, Chun Kit

PY - 2023/11

Y1 - 2023/11

N2 - Aptamers are single-stranded oligonucleotides that bind to their targets via specific structural interactions. To improve the properties and performance of aptamers, modified nucleotides are incorporated during or after a selection process such as systematic evolution of ligands by exponential enrichment (SELEX). We summarize the latest modified nucleotides and strategies used in modified (mod)-SELEX and post-SELEX to develop modified aptamers, highlight the methods used to characterize aptamer–target interactions, and present recent progress in modified aptamers that recognize different targets. We discuss the challenges and perspectives in further advancing the methodologies and toolsets to accelerate the discovery of modified aptamers, improve the throughput of aptamer-target characterization, and expand the functional diversity and complexity of modified aptamers. © 2023 Elsevier Ltd.

AB - Aptamers are single-stranded oligonucleotides that bind to their targets via specific structural interactions. To improve the properties and performance of aptamers, modified nucleotides are incorporated during or after a selection process such as systematic evolution of ligands by exponential enrichment (SELEX). We summarize the latest modified nucleotides and strategies used in modified (mod)-SELEX and post-SELEX to develop modified aptamers, highlight the methods used to characterize aptamer–target interactions, and present recent progress in modified aptamers that recognize different targets. We discuss the challenges and perspectives in further advancing the methodologies and toolsets to accelerate the discovery of modified aptamers, improve the throughput of aptamer-target characterization, and expand the functional diversity and complexity of modified aptamers. © 2023 Elsevier Ltd.

UR - https://www.scopus.com/pages/publications/85161583270

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U2 - 10.1016/j.tibtech.2023.05.005

DO - 10.1016/j.tibtech.2023.05.005

M3 - RGC 21 - Publication in refereed journal

SN - 0167-7799

VL - 41

SP - 1360

EP - 1384

JO - Trends in Biotechnology

JF - Trends in Biotechnology

IS - 11

ER -

Modified nucleic acid aptamers: development, characterization, and biological applications

Abstract

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