MiR-142-3p blocks TGF-β-induced activation of hepatic stellate cells through targeting TGFβRI

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Xiaoxue Yang
  • Ruoting Men
  • Liping Ma
  • Maoyao Wen
  • Yong Peng
  • Li Yang

Related Research Unit(s)

Detail(s)

Original languageEnglish
Pages (from-to)22-30
Journal / PublicationLife Sciences
Volume187
Online published18 Aug 2017
Publication statusPublished - 15 Oct 2017

Abstract

Aim: To understand the contribution of miR-142-3p in the activation of hepatic stellate cells (HSCs) and liver fibrosis, and the underlying mechanism. Materials and methods: We detected microRNAs expression profiles in quiescent and activated HSCs by microRNA-array, and performed qRT-PCR to validate these data in HSCs and plasma of cirrhosis patients. In vitro, the 3rd–5th passage HSCs was transfected with mir-142-3p mimics or stimulated with TGF β. The markers of HSCs activation (i.e. FN and α-SMA) were examined by qRT-PCR and western blotting, and cell viability was detected by MTT, colony formation assays respectively. Key finding: In our study, we identified miR-142-3p as a novel regulator of HSCs activation and indicator of hepatic cirrhosis. We found that miR-142-3p was significantly reduced in activated HSCs, while TGFβRI was distinctly up-regulated in activated HSCs. Ectopic expression of miR-142-3p in activated HSCs inhibited cell viability as well as cell growth, and blocked HSCs activation, concomitant with decreased transdifferentiation markers (i.e. FN and α-SMA). Further, we confirmed that miR-142-3p was reduced upon TGF-β exposure, while diminishing TGF-β-Smad signaling pathway in turn by reducing TGFβRI expression in HSCs. Besides, the plasma level of miR-142-3p declined significantly in patients with hepatic cirrhosis. Significance: In conclusion, we demonstrated that miR-142-3p repressed TGF-β-Smad signaling pathway to prevent HSCs activation through directly targeting TGFβRI in HSCs.

Research Area(s)

  • Hepatic stellate cells, Liver fibrosis, miR-142-3p, TGF-β-Smad signaling pathway, TGFβRI

Citation Format(s)

MiR-142-3p blocks TGF-β-induced activation of hepatic stellate cells through targeting TGFβRI. / Yang, Xiaoxue; Dan, Xuelian; Men, Ruoting et al.
In: Life Sciences, Vol. 187, 15.10.2017, p. 22-30.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review