Mink1 regulates β-catenin-independent wnt signaling via prickle phosphorylation

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • Avais M. Daulat
  • Olivia Luu
  • Anson Sing
  • Jeffrey L. Wrana
  • Helen Mcneill
  • Rudolf Winklbauer
  • Stéphane Angers

Detail(s)

Original languageEnglish
Pages (from-to)173-185
Journal / PublicationMolecular and Cellular Biology
Volume32
Issue number1
Publication statusPublished - Jan 2012
Externally publishedYes

Abstract

β-Catenin-independent Wnt signaling pathways have been implicated in the regulation of planar cell polarity (PCP) and convergent extension (CE) cell movements. Prickle, one of the core proteins of these pathways, is known to asymmetrically localize proximally at the adherens junction of Drosophila melanogaster wing cells and to locally accumulate within plasma membrane subdomains in cells undergoing CE movements during vertebrate development. Using mass spectrometry, we have identified the Ste20 kinase Mink1 as a Prickle-associated protein and found that they genetically interact during the establishment of PCP in the Drosophila eye and CE in Xenopus laevis embryos. We show that Mink1 phosphorylates Prickle on a conserved threonine residue and regulates its Rab5-dependent endosomal trafficking, a process required for the localized plasma membrane accumulation and function of Prickle. Mink1 also was found to be important for the clustering of Vangl within plasma membrane puncta. Our results provide a link between Mink and the Vangl-Prickle complex and highlight the importance of Prickle phosphorylation and endosomal trafficking for its function during Wnt-PCP signaling. © 2012, American Society for Microbiology.

Citation Format(s)

Mink1 regulates β-catenin-independent wnt signaling via prickle phosphorylation. / Daulat, Avais M.; Luu, Olivia; Sing, Anson; Zhang, Liang; Wrana, Jeffrey L.; Mcneill, Helen; Winklbauer, Rudolf; Angers, Stéphane.

In: Molecular and Cellular Biology, Vol. 32, No. 1, 01.2012, p. 173-185.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal