TY - GEN
T1 - Miniature RT-PCR systems integrated with a sample pretreatment device for virus detection
AU - Lien, Kang-Yi
AU - Lin, Wang-Ying
AU - Wang, Chih-Hao
AU - Lei, Huan-Yao
AU - Lee, Gwo-Bin
N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].
PY - 2007
Y1 - 2007
N2 - This paper presents a new chip-based reverse transcription polymerase chain reaction (RT-PCR) system integrated with a sample pretreatment device for fast DNA amplification and diagnosis of RNA-based viruses. A new two-way serpentine-shape (S-shape) pneumatic micropump and a magnetic bioseparator were developed for purification and enrichment of viruses. The target RNA-based virus would be bound onto the antibody-conjugated superparamagnetic beads, followed by collection and purification processes utilizing the on-chip bioseparator composed of microcoils. The new bioseparator/microreactor was designed for sample enrichment and bio-reaction. Then, the enriched target viruses can be used to perform fast nucleic acid amplification automatically in the RT-PCR chamber. The developed system may provide a powerful platform for sample pretreatment and fast diseases diagnosis. © 2007 IEEE.
AB - This paper presents a new chip-based reverse transcription polymerase chain reaction (RT-PCR) system integrated with a sample pretreatment device for fast DNA amplification and diagnosis of RNA-based viruses. A new two-way serpentine-shape (S-shape) pneumatic micropump and a magnetic bioseparator were developed for purification and enrichment of viruses. The target RNA-based virus would be bound onto the antibody-conjugated superparamagnetic beads, followed by collection and purification processes utilizing the on-chip bioseparator composed of microcoils. The new bioseparator/microreactor was designed for sample enrichment and bio-reaction. Then, the enriched target viruses can be used to perform fast nucleic acid amplification automatically in the RT-PCR chamber. The developed system may provide a powerful platform for sample pretreatment and fast diseases diagnosis. © 2007 IEEE.
UR - https://www.scopus.com/pages/publications/50149100375
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-50149100375&origin=recordpage
U2 - 10.1109/memsys.2007.4433018
DO - 10.1109/memsys.2007.4433018
M3 - RGC 32 - Refereed conference paper (with host publication)
SN - 1424409519
SN - 9781424409518
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 77
EP - 80
BT - Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007
T2 - 20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007
Y2 - 21 January 2007 through 25 January 2007
ER -