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Mimicking Dividing Cells by Assembly of Protein Structures Inside Aqueous Two-Phase Droplets

  • HC Shum
  • , Y Song
  • , T Knowles
  • , T Michaels

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review

Abstract

In this work, we demonstrate that assembly of macromolecules, such as proteins, can cause aqueous droplets to exhibit division, even in the absence of a cell membrane. The all-aqueous nature of the systems results in tunable interfacial tension, affinity partitioning and osmotic responses. The solubility of_x000D_ different types of macromolecules across the interfaces enables new strategies to assemble structures at the droplet interfaces. While the significantly lower interfacial tension can make stabilization of the interface difficult due to the slow adsorption dynamics by surfactants and particles, structures that have_x000D_ been assembled at the interfaces can be easily expelled. This contributes to the more sophisticated dynamics of the hierarhically structured all-aqueous droplets. These droplets have great potential to be utilized as templates for fabricating materials with novel properties.
Original languageEnglish
Title of host publicationMaterials Research Society (MRS) Fall Meeting 2018
PublisherMaterials Research Society
Publication statusPublished - 25 Nov 2018
Externally publishedYes
EventMaterials Research Society (MRS) Fall Meeting 2018 - Boston, United States
Duration: 25 Nov 201830 Nov 2018

Publication series

NameMaterials Research Society (MRS) Fall Meeting 2018

Conference

ConferenceMaterials Research Society (MRS) Fall Meeting 2018
PlaceUnited States
CityBoston
Period25/11/1830/11/18

Bibliographical note

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