Micromixing-Enhanced Biosensing of Radioactivity Using Modified Deinococcus Radiodurans in Microfluidics

Raymond H. W. Lam; Chengpeng Yang; Vincent W. T. Li; Wei Huang

doi:10.1109/NEMS.2019.8915638

Micromixing-Enhanced Biosensing of Radioactivity Using Modified Deinococcus Radiodurans in Microfluidics

Raymond H. W. Lam^*
, Chengpeng Yang
, Vincent W. T. Li
, Wei Huang

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

Abstract

α-radiation is extremely harmful if inhaled or swallowed, as it can be easily cause carcinogenetic or other physiological diseases. Here, we develop a microfluidic radiation biosensing device based on the β-galactosidase generation of genetically modified bacteria Deinococcus Radiodurans PZ0423 (DRPZ423) upon exposure of the radiation. An electrokinetic bacteria trapper is configured to locally aggregate bacteria to enhance the sensitivity. Additionally, a micro-Thick transparent silicone membrane (<20 μm) has been applied to contain the sensing bacteria with only an radiation reduction of <5 %. Moreover, a micro mixer was further integrated to shorten the sensing time and reduce the loss of p-Aminophenol. Altogether, this work has demonstrated a novel microfluidic radiation biosensing technique for achieving reliable α-radiation monitoring.

Original language	English
Title of host publication	Proceedings of the 14th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems
Publisher	IEEE
Pages	14-18
ISBN (Electronic)	978-1-7281-1629-7
ISBN (Print)	978-1-7281-1630-3
DOIs	https://doi.org/10.1109/NEMS.2019.8915638
Publication status	Published - Apr 2019
Event	14th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS 2019) - Bangkok, Thailand Duration: 11 Apr 2019 → 14 Apr 2019 https://ieee-nems.org/2019/

Publication series

Name	International Conference on Nano-Micro Engineered and Molecular Systems NEMS
ISSN (Print)	2474-3747
ISSN (Electronic)	2474-3755

Conference

Conference	14th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS 2019)
Place	Thailand
City	Bangkok
Period	11/04/19 → 14/04/19
Internet address	https://ieee-nems.org/2019/

Research Keywords

ALPHA-PARTICLES

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Lam, R. H. W., Yang, C., Li, V. W. T., & Huang, W. (2019). Micromixing-Enhanced Biosensing of Radioactivity Using Modified Deinococcus Radiodurans in Microfluidics. In Proceedings of the 14th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (pp. 14-18). Article 8915638 (International Conference on Nano-Micro Engineered and Molecular Systems NEMS). IEEE. https://doi.org/10.1109/NEMS.2019.8915638

Lam, Raymond H. W. ; Yang, Chengpeng ; Li, Vincent W. T. et al. / Micromixing-Enhanced Biosensing of Radioactivity Using Modified Deinococcus Radiodurans in Microfluidics. Proceedings of the 14th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems. IEEE, 2019. pp. 14-18 (International Conference on Nano-Micro Engineered and Molecular Systems NEMS).

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title = "Micromixing-Enhanced Biosensing of Radioactivity Using Modified Deinococcus Radiodurans in Microfluidics",

abstract = "α-radiation is extremely harmful if inhaled or swallowed, as it can be easily cause carcinogenetic or other physiological diseases. Here, we develop a microfluidic radiation biosensing device based on the β-galactosidase generation of genetically modified bacteria Deinococcus Radiodurans PZ0423 (DRPZ423) upon exposure of the radiation. An electrokinetic bacteria trapper is configured to locally aggregate bacteria to enhance the sensitivity. Additionally, a micro-Thick transparent silicone membrane (<20 μm) has been applied to contain the sensing bacteria with only an radiation reduction of <5 \%. Moreover, a micro mixer was further integrated to shorten the sensing time and reduce the loss of p-Aminophenol. Altogether, this work has demonstrated a novel microfluidic radiation biosensing technique for achieving reliable α-radiation monitoring.",

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Lam, RHW , Yang, C , Li, VWT & Huang, W 2019, Micromixing-Enhanced Biosensing of Radioactivity Using Modified Deinococcus Radiodurans in Microfluidics. in Proceedings of the 14th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems., 8915638, International Conference on Nano-Micro Engineered and Molecular Systems NEMS, IEEE, pp. 14-18, 14th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS 2019), Bangkok, Thailand, 11/04/19. https://doi.org/10.1109/NEMS.2019.8915638

Micromixing-Enhanced Biosensing of Radioactivity Using Modified Deinococcus Radiodurans in Microfluidics. / Lam, Raymond H. W.; Yang, Chengpeng ; Li, Vincent W. T. et al.
Proceedings of the 14th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems. IEEE, 2019. p. 14-18 8915638 (International Conference on Nano-Micro Engineered and Molecular Systems NEMS).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

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T1 - Micromixing-Enhanced Biosensing of Radioactivity Using Modified Deinococcus Radiodurans in Microfluidics

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AU - Li, Vincent W. T.

AU - Huang, Wei

PY - 2019/4

Y1 - 2019/4

N2 - α-radiation is extremely harmful if inhaled or swallowed, as it can be easily cause carcinogenetic or other physiological diseases. Here, we develop a microfluidic radiation biosensing device based on the β-galactosidase generation of genetically modified bacteria Deinococcus Radiodurans PZ0423 (DRPZ423) upon exposure of the radiation. An electrokinetic bacteria trapper is configured to locally aggregate bacteria to enhance the sensitivity. Additionally, a micro-Thick transparent silicone membrane (<20 μm) has been applied to contain the sensing bacteria with only an radiation reduction of <5 %. Moreover, a micro mixer was further integrated to shorten the sensing time and reduce the loss of p-Aminophenol. Altogether, this work has demonstrated a novel microfluidic radiation biosensing technique for achieving reliable α-radiation monitoring.

AB - α-radiation is extremely harmful if inhaled or swallowed, as it can be easily cause carcinogenetic or other physiological diseases. Here, we develop a microfluidic radiation biosensing device based on the β-galactosidase generation of genetically modified bacteria Deinococcus Radiodurans PZ0423 (DRPZ423) upon exposure of the radiation. An electrokinetic bacteria trapper is configured to locally aggregate bacteria to enhance the sensitivity. Additionally, a micro-Thick transparent silicone membrane (<20 μm) has been applied to contain the sensing bacteria with only an radiation reduction of <5 %. Moreover, a micro mixer was further integrated to shorten the sensing time and reduce the loss of p-Aminophenol. Altogether, this work has demonstrated a novel microfluidic radiation biosensing technique for achieving reliable α-radiation monitoring.

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M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 978-1-7281-1630-3

T3 - International Conference on Nano-Micro Engineered and Molecular Systems NEMS

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Y2 - 11 April 2019 through 14 April 2019

ER -

Lam RHW , Yang C , Li VWT , Huang W. Micromixing-Enhanced Biosensing of Radioactivity Using Modified Deinococcus Radiodurans in Microfluidics. In Proceedings of the 14th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems. IEEE. 2019. p. 14-18. 8915638. (International Conference on Nano-Micro Engineered and Molecular Systems NEMS). doi: 10.1109/NEMS.2019.8915638

Micromixing-Enhanced Biosensing of Radioactivity Using Modified Deinococcus Radiodurans in Microfluidics

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