Micromachined flow-through polymerase chain reaction chip utilizing multiple membrane-activated micropumps

Chih-Hao Wang; Yi-Yu Chen; Chia-Sheng Liao; Huei-Huang Lee; Gwo-Bin Lee

doi:10.1109/memsys.2006.1627814

Micromachined flow-through polymerase chain reaction chip utilizing multiple membrane-activated micropumps

Chih-Hao Wang
, Yi-Yu Chen
, Chia-Sheng Liao
, Huei-Huang Lee
, Gwo-Bin Lee

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

3 Citations (Scopus)

Abstract

This paper reports a new micromachined flow-through polymerase chain reaction (PCR) chip for applications of rapid pathogen diagnosis. The PCR chip comprised a micro thermal control module and a microfluidic control module fabricated using MEMS (micro-electro-mechanical-systems) technology. The micro thermal control module was formed with three individual heating and temperature-sensing sections, each modulating a specific temperature for denaturation, annealing and extension process, respectively. The membrane-activated micropumps were used to transport sample fluids through three reaction regions to adjust the time ratio and cycle numbers for PCR. The experimental results showed that S. pneumoniae detection gene (273 bps) could be amplified successfully using the new flow-through PCR chip. The new PCR chip could be promising for rapid clinical diagnosis of DNA-based infectious disease. © 2006 IEEE.

Original language	English
Title of host publication	19th IEEE International Conference on Micro Electro Mechanical Systems
Pages	374-377
Volume	2006
DOIs	https://doi.org/10.1109/memsys.2006.1627814
Publication status	Published - 2006
Externally published	Yes
Event	19th IEEE International Conference on Micro Electro Mechanical Systems - Istanbul, Türkiye Duration: 22 Jan 2006 → 26 Jan 2006

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume	2006
ISSN (Print)	1084-6999

Conference

Conference	19th IEEE International Conference on Micro Electro Mechanical Systems
Place	Türkiye
City	Istanbul
Period	22/01/06 → 26/01/06

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1109/memsys.2006.1627814

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Wang, C.-H., Chen, Y.-Y., Liao, C.-S., Lee, H.-H., & Lee, G.-B. (2006). Micromachined flow-through polymerase chain reaction chip utilizing multiple membrane-activated micropumps. In 19th IEEE International Conference on Micro Electro Mechanical Systems (Vol. 2006, pp. 374-377). Article 1627814 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2006). https://doi.org/10.1109/memsys.2006.1627814

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title = "Micromachined flow-through polymerase chain reaction chip utilizing multiple membrane-activated micropumps",

abstract = "This paper reports a new micromachined flow-through polymerase chain reaction (PCR) chip for applications of rapid pathogen diagnosis. The PCR chip comprised a micro thermal control module and a microfluidic control module fabricated using MEMS (micro-electro-mechanical-systems) technology. The micro thermal control module was formed with three individual heating and temperature-sensing sections, each modulating a specific temperature for denaturation, annealing and extension process, respectively. The membrane-activated micropumps were used to transport sample fluids through three reaction regions to adjust the time ratio and cycle numbers for PCR. The experimental results showed that S. pneumoniae detection gene (273 bps) could be amplified successfully using the new flow-through PCR chip. The new PCR chip could be promising for rapid clinical diagnosis of DNA-based infectious disease. {\textcopyright} 2006 IEEE.",

author = "Chih-Hao Wang and Yi-Yu Chen and Chia-Sheng Liao and Huei-Huang Lee and Gwo-Bin Lee",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].; 19th IEEE International Conference on Micro Electro Mechanical Systems ; Conference date: 22-01-2006 Through 26-01-2006",

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Wang, C-H, Chen, Y-Y, Liao, C-S, Lee, H-H & Lee, G-B 2006, Micromachined flow-through polymerase chain reaction chip utilizing multiple membrane-activated micropumps. in 19th IEEE International Conference on Micro Electro Mechanical Systems. vol. 2006, 1627814, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), vol. 2006, pp. 374-377, 19th IEEE International Conference on Micro Electro Mechanical Systems, Istanbul, Türkiye, 22/01/06. https://doi.org/10.1109/memsys.2006.1627814

Micromachined flow-through polymerase chain reaction chip utilizing multiple membrane-activated micropumps. / Wang, Chih-Hao; Chen, Yi-Yu; Liao, Chia-Sheng et al.
19th IEEE International Conference on Micro Electro Mechanical Systems. Vol. 2006 2006. p. 374-377 1627814 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2006).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - Micromachined flow-through polymerase chain reaction chip utilizing multiple membrane-activated micropumps

AU - Wang, Chih-Hao

AU - Chen, Yi-Yu

AU - Liao, Chia-Sheng

AU - Lee, Huei-Huang

AU - Lee, Gwo-Bin

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2006

Y1 - 2006

N2 - This paper reports a new micromachined flow-through polymerase chain reaction (PCR) chip for applications of rapid pathogen diagnosis. The PCR chip comprised a micro thermal control module and a microfluidic control module fabricated using MEMS (micro-electro-mechanical-systems) technology. The micro thermal control module was formed with three individual heating and temperature-sensing sections, each modulating a specific temperature for denaturation, annealing and extension process, respectively. The membrane-activated micropumps were used to transport sample fluids through three reaction regions to adjust the time ratio and cycle numbers for PCR. The experimental results showed that S. pneumoniae detection gene (273 bps) could be amplified successfully using the new flow-through PCR chip. The new PCR chip could be promising for rapid clinical diagnosis of DNA-based infectious disease. © 2006 IEEE.

AB - This paper reports a new micromachined flow-through polymerase chain reaction (PCR) chip for applications of rapid pathogen diagnosis. The PCR chip comprised a micro thermal control module and a microfluidic control module fabricated using MEMS (micro-electro-mechanical-systems) technology. The micro thermal control module was formed with three individual heating and temperature-sensing sections, each modulating a specific temperature for denaturation, annealing and extension process, respectively. The membrane-activated micropumps were used to transport sample fluids through three reaction regions to adjust the time ratio and cycle numbers for PCR. The experimental results showed that S. pneumoniae detection gene (273 bps) could be amplified successfully using the new flow-through PCR chip. The new PCR chip could be promising for rapid clinical diagnosis of DNA-based infectious disease. © 2006 IEEE.

UR - https://www.scopus.com/pages/publications/33750129514

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-33750129514&origin=recordpage

U2 - 10.1109/memsys.2006.1627814

DO - 10.1109/memsys.2006.1627814

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 0780394755

SN - 9780780394759

VL - 2006

T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

SP - 374

EP - 377

BT - 19th IEEE International Conference on Micro Electro Mechanical Systems

T2 - 19th IEEE International Conference on Micro Electro Mechanical Systems

Y2 - 22 January 2006 through 26 January 2006

ER -

Wang CH, Chen YY, Liao CS, Lee HH, Lee GB. Micromachined flow-through polymerase chain reaction chip utilizing multiple membrane-activated micropumps. In 19th IEEE International Conference on Micro Electro Mechanical Systems. Vol. 2006. 2006. p. 374-377. 1627814. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/memsys.2006.1627814

Micromachined flow-through polymerase chain reaction chip utilizing multiple membrane-activated micropumps

Abstract

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Conference

Bibliographical note

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