Microliter Sample Insulin Detection Using a Screen-Printed Electrode Modified by Nickel Hydroxide

The monitoring of insulin, which is the only hormone that helps regulate blood glucose levels in the body, plays a key role in the diagnosis and treatment of diabetes. However, most techniques today involve complicated electrode fabrication and testing processes, which are time-consuming and costly, and require a relatively large volume of sample. To overcome these drawbacks, we present here a low-cost insulin detection method based on a screen-printed electrode (SPE) modified by nickel hydroxide (Ni(OH)₂). This novel method only requires 300 μL of insulin sample, and the time it takes for electrode preparation is about 12 times shorter than traditional electrode fabrication methods such as coating and sol-gel methods. The electrochemical behaviors of the Ni(OH)₂-coated SPE (NSPE) sensing area in insulin aqueous solutions are studied using cyclic voltammetry, amperometric i-t curves, and electrochemical impedance spectroscopy. The results demonstrate that the NSPE sensing surface has excellent detection properties, such as a high sensitivity of 15.3 μA·μM^-1 and a low detection limit of 138 nM. It takes a short time (∼10 min) to prepare the NSPE sensing surface, and only two drops (∼300 μL) of insulin samples are required in the detection process. Moreover, the selectivity of this method for insulin detection is verified by detecting mixtures of insulin and ascorbic acid or bovine hemoglobin. Finally, we discuss the potential clinical applications of this method by detecting various concentrations of insulin in human serum.