Microfluidic Technology for Nucleic Acid Aptamer Evolution and Application

Lewis A. Fraser; Yee-Wai Cheung; Andrew B. Kinghorn; Wei Guo; Simon Chi-Chin Shiu; Chandra Jinata; Mengping Liu; Soubhagya Bhuyan; Lang Nan; Ho Cheung Shum; Julian A. Tanner

doi:10.1002/adbi.201900012

Microfluidic Technology for Nucleic Acid Aptamer Evolution and Application

Lewis A. Fraser
, Yee-Wai Cheung
, Andrew B. Kinghorn
, Wei Guo
, Simon Chi-Chin Shiu
, Chandra Jinata
, Mengping Liu
, Soubhagya Bhuyan
, Lang Nan
, Ho Cheung Shum^*
, Julian A. Tanner

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

31 Citations (Scopus)

Abstract

The intersection of microfluidics and aptamer technologies holds particular promise for rapid progress in a plethora of applications across biomedical science and other areas. Here, the influence of microfluidics on the field of aptamers, from traditional capillary electrophoresis approaches through innovative modern-day approaches using micromagnetic beads and emulsion droplets, is reviewed. Miniaturizing aptamer-based bioassays through microfluidics has the potential to transform diagnostics and embedded biosensing in the coming years. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Original language	English
Article number	1900012
Journal	Advanced Biosystems
Volume	3
Issue number	5
DOIs	https://doi.org/10.1002/adbi.201900012
Publication status	Published - 1 May 2019
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Funding

This work was supported by Hong Kong University Grants Council under Research Grants Council (RGC) General Research Fund (GRF) grants 17163416 and 17127515 to J.A.T., and under General Research Fund (GRF) grants 17304514 and 17329516 to H.C.S.

Research Keywords

aptamers
biosensors
microfluidics
selection
SELEX

RGC Funding Information

RGC-funded

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10.1002/adbi.201900012

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AU - Fraser, Lewis A.

AU - Cheung, Yee-Wai

AU - Kinghorn, Andrew B.

AU - Guo, Wei

AU - Shiu, Simon Chi-Chin

AU - Jinata, Chandra

AU - Liu, Mengping

AU - Bhuyan, Soubhagya

AU - Nan, Lang

AU - Shum, Ho Cheung

AU - Tanner, Julian A.

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AB - The intersection of microfluidics and aptamer technologies holds particular promise for rapid progress in a plethora of applications across biomedical science and other areas. Here, the influence of microfluidics on the field of aptamers, from traditional capillary electrophoresis approaches through innovative modern-day approaches using micromagnetic beads and emulsion droplets, is reviewed. Miniaturizing aptamer-based bioassays through microfluidics has the potential to transform diagnostics and embedded biosensing in the coming years. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

KW - aptamers

KW - biosensors

KW - microfluidics

KW - selection

KW - SELEX

UR - https://www.scopus.com/pages/publications/85063957455

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U2 - 10.1002/adbi.201900012

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SN - 2366-7478

VL - 3

JO - Advanced Biosystems

JF - Advanced Biosystems

IS - 5

M1 - 1900012

ER -

Microfluidic Technology for Nucleic Acid Aptamer Evolution and Application

Abstract

Bibliographical note

Funding

Research Keywords

RGC Funding Information

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