Microfluidic studies of hydrostatic pressure-enhanced doxorubicin resistance in human breast cancer cells

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

4 Scopus Citations
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Author(s)

  • Menglin Shang
  • Su Bin Lim
  • Kuan Jiang
  • Yoon Sim Yap
  • Jongyoon Han
  • Chwee Teck Lim

Related Research Unit(s)

Detail(s)

Original languageEnglish
Pages (from-to)746-754
Journal / PublicationLab on a Chip
Volume21
Issue number4
Online published20 Jan 2021
Publication statusPublished - 21 Feb 2021

Abstract

Acquired multidrug resistance in tumors is a big challenge in cancer therapy. As an important physical stimulus in the tumor microenvironment, elevated interstitial fluid pressure has been reported to inhibit drug delivery and promote metastasis in solid tumors. However, the direct influence of this fluid pressure on anticancer drug resistance remains unclear. Here, we develop a pressurized in vitro circulating tumor cell (CTC) culture platform for anticancer drug screening. By using human breast cancer cell line MCF-7 and MDA-MB-231, we find that doxorubicin resistance can be increased by up to 2.5 times under 30 mmHg culture condition, through ABCC1 overexpression that reduces intracellular doxorubicin concentration. A similar chemoresistance change is also observed in clinical metastatic circulating tumor cells samples. These findings provide a new insight into the chemoresistance mechanism of metastatic human breast cancer cells and elucidate the significance of abnormal hydrostatic pressure in cancer progression.

Citation Format(s)

Microfluidic studies of hydrostatic pressure-enhanced doxorubicin resistance in human breast cancer cells. / Shang, Menglin; Lim, Su Bin; Jiang, Kuan; Yap, Yoon Sim; Khoo, Bee Luan; Han, Jongyoon; Lim, Chwee Teck.

In: Lab on a Chip, Vol. 21, No. 4, 21.02.2021, p. 746-754.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review