Microfluidic platform for probing cancer cells migration property under periodic mechanical confinement

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Ran Wang
  • Shuxun Chen
  • Yu-Ting Chow

Detail(s)

Original languageEnglish
Article number024118
Journal / PublicationBiomicrofluidics
Volume12
Issue number2
Online published27 Apr 2018
Publication statusPublished - 2018

Link(s)

Abstract

Cancer cell migration and invasion, which are involved in tumour metastasis, are hard to predict and control. Numerous studies have demonstrated that physical cues influence cancer cell migration and affect tumour metastasis. In this study, we proposed the use of a microchannel chip equipped with a number of vertical constrictions to produce periodic compression forces on cells passing through narrow channels. The chip with repeated vertical confinement was applied on adherent MHCC-97L liver cancer cells and suspended OCI-AML leukaemia cells to determine the migration ability of these cancer cells. Given the stimulation of the periodic mechanical confinement on-chip, the migration ability of cancer cells was promoted. Moreover, the migration speed increased as the stimulation was enhanced. Both AFM nanoindentation and optical stretching tests on cancer cells were performed to measure their mechanical property. After confinement stimulation, the cancer cells possessed higher deformability and lower stiffness than non-stimulating cells. The confinement stimulation altered the cell cytoskeleton, which governs the migration speed. This phenomenon was determined through gene expression analysis. The proposed on-chip cell migration assays will help characterise the migration property of cancer cells and benefit the development of new therapeutic strategies for metastasis.

Research Area(s)

Citation Format(s)

Microfluidic platform for probing cancer cells migration property under periodic mechanical confinement. / Ma, Dongce; Wang, Ran; Chen, Shuxun et al.
In: Biomicrofluidics, Vol. 12, No. 2, 024118, 2018.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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