Microfluidic particle dam for direct visualization of SARS-CoV-2 antibody levels in COVID-19 vaccinees
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Author(s)
Related Research Unit(s)
Detail(s)
Original language | English |
---|---|
Article number | eabn6064 |
Journal / Publication | Science Advances |
Volume | 8 |
Issue number | 22 |
Online published | 3 Jun 2022 |
Publication status | Published - 3 Jun 2022 |
Link(s)
DOI | DOI |
---|---|
Attachment(s) | Documents
Publisher's Copyright Statement
|
Link to Scopus | https://www.scopus.com/record/display.uri?eid=2-s2.0-85131702227&origin=recordpage |
Permanent Link | https://scholars.cityu.edu.hk/en/publications/publication(05cd6267-08b5-4709-bfc4-18bf40de33c8).html |
Abstract
Various COVID-19 vaccines are currently deployed, but their immunization varies and decays with time. Antibody level is a potent correlate to immune protection, but its quantitation relies on intensive laboratory techniques. Here, we report a decentralized, instrument-free microfluidic device that directly visualizes SARS-CoV-2 antibody levels. Magnetic microparticles (MMPs) and polystyrene microparticles (PMPs) can bind to SARS- CoV-2 antibodies simultaneously. In a microfluidic chip, this binding reduces the incidence of free PMPs escaping from magnetic separation and shortens PMP accumulation length at a particle dam. This visual quantitative result enables use in either sensitive mode [limit of detection (LOD): 13.3 ng/ml; sample-to-answer time: 70 min] or rapid mode (LOD: 57.8 ng/ml; sample-to-answer time: 20 min) and closely agrees with the gold standard enzyme-linked immunosorbent assay. Trials on 91 vaccinees revealed higher antibody levels in mRNA vaccinees than in inactivated vaccinees and their decay in 45 days, demonstrating the need for point-of-care devices to monitor immune protection.
Research Area(s)
- DETECTION LIMITS, QUANTITATIVE DETECTION
Citation Format(s)
Microfluidic particle dam for direct visualization of SARS-CoV-2 antibody levels in COVID-19 vaccinees. / Wu, Minghui; Wu, Siying; Wang, Gaobo et al.
In: Science Advances, Vol. 8, No. 22, eabn6064, 03.06.2022.
In: Science Advances, Vol. 8, No. 22, eabn6064, 03.06.2022.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Download Statistics
No data available