MICROFLUIDIC ISOLATION OF SINGLE CELLS USING ARC-EDGE-CROSS-SECTION MONOLITHIC VALVES FOR GENE MUTATION HETEROGENEITY ANALYSIS

Tao Xu; Huayang Fu; Mengsu Yang

MICROFLUIDIC ISOLATION OF SINGLE CELLS USING ARC-EDGE-CROSS-SECTION MONOLITHIC VALVES FOR GENE MUTATION HETEROGENEITY ANALYSIS

Tao Xu, Huayang Fu, Mengsu Yang^*

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

Abstract

We developed a simple technique to fabricate arc-edge-channel monolithic valves (AMVs) in microfluidic chips. The AMVs were used in a microfluidic device for parallel liquid handling and single cell isolation. We also developed an allele-specific PCR assay for detecting common mutations in EGFR, KRAS and BRAF genes at single-cell level. The results show that mutation heterogeneity exists in lung adenocarcinoma cell lines. The detection of both single (T790M) and double (L858R and T790M) mutations in cancer cells isolated from a clinical pleural effusion sample revealed the association of drug-resistance status with T790M mutation.

Original language	English
Title of host publication	μTAS 2017
Subtitle of host publication	The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	1619-1620
ISBN (Electronic)	9780692941836
ISBN (Print)	9780692941836
Publication status	Published - Oct 2017
Event	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017) - Savannah, United States Duration: 22 Oct 2017 → 26 Oct 2017 https://archive.cbmsociety.org/utas/uTAS2017.zip

Publication series

Name	International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS
Publisher	Chemical and Biological Microsystems Society
ISSN (Electronic)	1556-5904

Conference

Conference	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017)
Place	United States
City	Savannah
Period	22/10/17 → 26/10/17
Internet address	https://archive.cbmsociety.org/utas/uTAS2017.zip

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Keywords

Monolithic valves
Mutation detection
Single-cell analysis
Tumor heterogeneity

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Xu, T., Fu, H., & Yang, M. (2017). MICROFLUIDIC ISOLATION OF SINGLE CELLS USING ARC-EDGE-CROSS-SECTION MONOLITHIC VALVES FOR GENE MUTATION HETEROGENEITY ANALYSIS. In μTAS 2017: The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1619-1620). (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS). Chemical and Biological Microsystems Society.

Xu, Tao ; Fu, Huayang ; Yang, Mengsu. / MICROFLUIDIC ISOLATION OF SINGLE CELLS USING ARC-EDGE-CROSS-SECTION MONOLITHIC VALVES FOR GENE MUTATION HETEROGENEITY ANALYSIS. μTAS 2017: The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2017. pp. 1619-1620 (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS).

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abstract = "We developed a simple technique to fabricate arc-edge-channel monolithic valves (AMVs) in microfluidic chips. The AMVs were used in a microfluidic device for parallel liquid handling and single cell isolation. We also developed an allele-specific PCR assay for detecting common mutations in EGFR, KRAS and BRAF genes at single-cell level. The results show that mutation heterogeneity exists in lung adenocarcinoma cell lines. The detection of both single (T790M) and double (L858R and T790M) mutations in cancer cells isolated from a clinical pleural effusion sample revealed the association of drug-resistance status with T790M mutation.",

keywords = "Monolithic valves, Mutation detection, Single-cell analysis, Tumor heterogeneity",

author = "Tao Xu and Huayang Fu and Mengsu Yang",

year = "2017",

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isbn = "9780692941836",

series = "International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS",

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booktitle = "μTAS 2017",

note = "21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017) ; Conference date: 22-10-2017 Through 26-10-2017",

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Xu, T, Fu, H & Yang, M 2017, MICROFLUIDIC ISOLATION OF SINGLE CELLS USING ARC-EDGE-CROSS-SECTION MONOLITHIC VALVES FOR GENE MUTATION HETEROGENEITY ANALYSIS. in μTAS 2017: The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS, Chemical and Biological Microsystems Society, pp. 1619-1620, 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017), Savannah, Georgia, United States, 22/10/17.

MICROFLUIDIC ISOLATION OF SINGLE CELLS USING ARC-EDGE-CROSS-SECTION MONOLITHIC VALVES FOR GENE MUTATION HETEROGENEITY ANALYSIS. / Xu, Tao; Fu, Huayang; Yang, Mengsu.
μTAS 2017: The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2017. p. 1619-1620 (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - MICROFLUIDIC ISOLATION OF SINGLE CELLS USING ARC-EDGE-CROSS-SECTION MONOLITHIC VALVES FOR GENE MUTATION HETEROGENEITY ANALYSIS

AU - Xu, Tao

AU - Fu, Huayang

AU - Yang, Mengsu

PY - 2017/10

Y1 - 2017/10

N2 - We developed a simple technique to fabricate arc-edge-channel monolithic valves (AMVs) in microfluidic chips. The AMVs were used in a microfluidic device for parallel liquid handling and single cell isolation. We also developed an allele-specific PCR assay for detecting common mutations in EGFR, KRAS and BRAF genes at single-cell level. The results show that mutation heterogeneity exists in lung adenocarcinoma cell lines. The detection of both single (T790M) and double (L858R and T790M) mutations in cancer cells isolated from a clinical pleural effusion sample revealed the association of drug-resistance status with T790M mutation.

AB - We developed a simple technique to fabricate arc-edge-channel monolithic valves (AMVs) in microfluidic chips. The AMVs were used in a microfluidic device for parallel liquid handling and single cell isolation. We also developed an allele-specific PCR assay for detecting common mutations in EGFR, KRAS and BRAF genes at single-cell level. The results show that mutation heterogeneity exists in lung adenocarcinoma cell lines. The detection of both single (T790M) and double (L858R and T790M) mutations in cancer cells isolated from a clinical pleural effusion sample revealed the association of drug-resistance status with T790M mutation.

KW - Monolithic valves

KW - Mutation detection

KW - Single-cell analysis

KW - Tumor heterogeneity

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UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85079677476&origin=recordpage

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9780692941836

T3 - International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS

SP - 1619

EP - 1620

BT - μTAS 2017

PB - Chemical and Biological Microsystems Society

T2 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017)

Y2 - 22 October 2017 through 26 October 2017

ER -

Xu T, Fu H, Yang M. MICROFLUIDIC ISOLATION OF SINGLE CELLS USING ARC-EDGE-CROSS-SECTION MONOLITHIC VALVES FOR GENE MUTATION HETEROGENEITY ANALYSIS. In μTAS 2017: The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2017. p. 1619-1620. (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS).

MICROFLUIDIC ISOLATION OF SINGLE CELLS USING ARC-EDGE-CROSS-SECTION MONOLITHIC VALVES FOR GENE MUTATION HETEROGENEITY ANALYSIS

Abstract

Publication series

Conference

UN SDGs

Research Keywords

Other Access Options

Permanent Link

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