MICROFLUIDIC CLUSTER ASSAY FOR PATIENT-DERIVED CIRCULATING TUMOR CELLS WITH RELEVANCE IN PATIENT PROGNOSIS

B.L. Khoo; G. Grenci; T. Jing; Y.B. Lim; S.C. Lee; J.P. Thiery; J. Han; C.T. Lim

MICROFLUIDIC CLUSTER ASSAY FOR PATIENT-DERIVED CIRCULATING TUMOR CELLS WITH RELEVANCE IN PATIENT PROGNOSIS

B.L. Khoo^*
, G. Grenci
, T. Jing
, Y.B. Lim
, S.C. Lee
, J.P. Thiery
, J. Han
, C.T. Lim

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

Abstract

We developed a novel circulating tumor cell (CTC) cluster microfluidic assay designed to mimic the in vivo tumor microenvironment for the label-free expansion of CTCs from clinically relevant blood volumes. Using specialized tapered microwells which promotes and mimics confined fluidic niche, we successfully expanded>50% of clinical blood samples (n=73) to achieve CTC clusters within two weeks. Putative CTCs may be presented throughout the cluster as single cells or aggregated microemboli, and could be detected with a panel of downstream analysis. The assay demonstrates superior performance in generating positive cultures over previously reported studies (<20%).

Original language	English
Title of host publication	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)
Publisher	Chemical and Biological Microsystems Society
Pages	280-282
Volume	1
ISBN (Electronic)	9780979806490
ISBN (Print)	9781510834163
Publication status	Published - Oct 2016
Externally published	Yes
Event	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016) - Dublin, Ireland Duration: 9 Oct 2016 → 13 Oct 2016

Conference

Conference	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)
Place	Ireland
City	Dublin
Period	9/10/16 → 13/10/16

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Keywords

Circulating tumor cells
CTC cluster
Drug response
Microfluidics
Microwells

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Khoo, B. L., Grenci, G., Jing, T., Lim, Y. B., Lee, S. C., Thiery, J. P., Han, J., & Lim, C. T. (2016). MICROFLUIDIC CLUSTER ASSAY FOR PATIENT-DERIVED CIRCULATING TUMOR CELLS WITH RELEVANCE IN PATIENT PROGNOSIS. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016) (Vol. 1, pp. 280-282). Chemical and Biological Microsystems Society.

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title = "MICROFLUIDIC CLUSTER ASSAY FOR PATIENT-DERIVED CIRCULATING TUMOR CELLS WITH RELEVANCE IN PATIENT PROGNOSIS",

abstract = "We developed a novel circulating tumor cell (CTC) cluster microfluidic assay designed to mimic the in vivo tumor microenvironment for the label-free expansion of CTCs from clinically relevant blood volumes. Using specialized tapered microwells which promotes and mimics confined fluidic niche, we successfully expanded>50\% of clinical blood samples (n=73) to achieve CTC clusters within two weeks. Putative CTCs may be presented throughout the cluster as single cells or aggregated microemboli, and could be detected with a panel of downstream analysis. The assay demonstrates superior performance in generating positive cultures over previously reported studies (<20\%).",

keywords = "Circulating tumor cells, CTC cluster, Drug response, Microfluidics, Microwells",

author = "B.L. Khoo and G. Grenci and T. Jing and Y.B. Lim and S.C. Lee and J.P. Thiery and J. Han and C.T. Lim",

year = "2016",

month = oct,

language = "English",

isbn = "9781510834163",

volume = "1",

pages = "280--282",

booktitle = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)",

publisher = "Chemical and Biological Microsystems Society",

note = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016) ; Conference date: 09-10-2016 Through 13-10-2016",

}

Khoo, BL, Grenci, G, Jing, T, Lim, YB, Lee, SC, Thiery, JP, Han, J & Lim, CT 2016, MICROFLUIDIC CLUSTER ASSAY FOR PATIENT-DERIVED CIRCULATING TUMOR CELLS WITH RELEVANCE IN PATIENT PROGNOSIS. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016). vol. 1, Chemical and Biological Microsystems Society, pp. 280-282, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016), Dublin, Ireland, 9/10/16.

MICROFLUIDIC CLUSTER ASSAY FOR PATIENT-DERIVED CIRCULATING TUMOR CELLS WITH RELEVANCE IN PATIENT PROGNOSIS. / Khoo, B.L.; Grenci, G.; Jing, T. et al.
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016). Vol. 1 Chemical and Biological Microsystems Society, 2016. p. 280-282.

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - MICROFLUIDIC CLUSTER ASSAY FOR PATIENT-DERIVED CIRCULATING TUMOR CELLS WITH RELEVANCE IN PATIENT PROGNOSIS

AU - Khoo, B.L.

AU - Grenci, G.

AU - Jing, T.

AU - Lim, Y.B.

AU - Lee, S.C.

AU - Thiery, J.P.

AU - Han, J.

AU - Lim, C.T.

PY - 2016/10

Y1 - 2016/10

N2 - We developed a novel circulating tumor cell (CTC) cluster microfluidic assay designed to mimic the in vivo tumor microenvironment for the label-free expansion of CTCs from clinically relevant blood volumes. Using specialized tapered microwells which promotes and mimics confined fluidic niche, we successfully expanded>50% of clinical blood samples (n=73) to achieve CTC clusters within two weeks. Putative CTCs may be presented throughout the cluster as single cells or aggregated microemboli, and could be detected with a panel of downstream analysis. The assay demonstrates superior performance in generating positive cultures over previously reported studies (<20%).

AB - We developed a novel circulating tumor cell (CTC) cluster microfluidic assay designed to mimic the in vivo tumor microenvironment for the label-free expansion of CTCs from clinically relevant blood volumes. Using specialized tapered microwells which promotes and mimics confined fluidic niche, we successfully expanded>50% of clinical blood samples (n=73) to achieve CTC clusters within two weeks. Putative CTCs may be presented throughout the cluster as single cells or aggregated microemboli, and could be detected with a panel of downstream analysis. The assay demonstrates superior performance in generating positive cultures over previously reported studies (<20%).

KW - Circulating tumor cells

KW - CTC cluster

KW - Drug response

KW - Microfluidics

KW - Microwells

UR - https://www.scopus.com/pages/publications/85014286021

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85014286021&origin=recordpage

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781510834163

VL - 1

SP - 280

EP - 282

BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)

PB - Chemical and Biological Microsystems Society

T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)

Y2 - 9 October 2016 through 13 October 2016

ER -

MICROFLUIDIC CLUSTER ASSAY FOR PATIENT-DERIVED CIRCULATING TUMOR CELLS WITH RELEVANCE IN PATIENT PROGNOSIS

Abstract

Conference

UN SDGs

Research Keywords

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