@inproceedings{327767f256a444f397d13584a6da5788,
title = "MICROFLUIDIC BUFFER EXCHANGE FOR INTERFERENCE-FREE MICRO/NANOPARTICLE CELL ENGINEERING",
abstract = "Cell labeling with active-ingredient-containing micro/nanoparticles (NPs) can enhance native therapeutic properties, enable bioimaging and control cell phenotype. Herein, we report an inertial microfluid-ics-based buffer exchange strategy to efficiently separate labeled cells from free NPs that inadvertently generate signals and impart transformative effects on neighboring non-target cells. We demonstrate restoration of inflammatory processes in the proximal microenvironment by removing free NPs loaded with anti-inflammatory steroids (dexamethasone) from engineered mesenchymal stem cells (MSCs). This single-step cell purification strategy enables high cell processing throughput (106 cells/min) and would be highly useful in large volume cell purification typically required in regenerative medicine.",
keywords = "Buffer exchange, Cell labeling, Nanoparticles",
author = "David Yeo and Christian Wiraja and Yingying Zhou and Huimin Tay and Chenjie Xu and Hanwei Hou",
year = "2015",
month = oct,
language = "English",
isbn = "9780979806483",
series = "MicroTAS - International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Chemical and Biological Microsystems Society",
pages = "540--542",
booktitle = "19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)",
note = "19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015), MicroTAS 2015 ; Conference date: 25-10-2015 Through 29-10-2015",
}