Microfluidic biosensing of viscoelastic properties of normal and cancerous human breast cells

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review

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Detail(s)

Original languageEnglish
Title of host publication2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)
PublisherIEEE
Pages90-95
ISBN (Electronic)978-1-5090-3059-0
Publication statusPublished - Apr 2017

Conference

Title12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
LocationUCLA
PlaceUnited States
CityLos Angeles
Period9 - 12 April 2017

Abstract

Biomechanical properties have been revealed as potential biomarkers for distinguishing cancer cells from normal cells. In this work, we report a novel technique using a confining microchannel embedded with microelectrodes for biomechanical phenotyping for floating human cells, including one normal breast cell line (MCF-10A) and two breast cancer cell lines (MCF-7 and MDA-MB-231). The floating cells move under a defined pressure profile along the microchannel, in which the cells deform dynamically under compression by the channel sidewalls. We adopt Hertz and Tatara models to convert the deformed cell shapes to cell diameters and transient stress-strain ratios. By further considering the cell viscoelasticity as a Standard Linear Solid (SLS) model, we compute for whole-cell viscosity, and instantaneous and relaxed moduli. Our results show that the selected cell types have significant different viscoelastic properties. Applications of the electrode-embedded confining microchannel can achieve high-Throughput, continuous-flow deep phenotyping of rare cells by functionalizing channel side walls with antibodies for both biomechanical and biochemical biomarkers for more comprehensive and promising cell characterization.

Citation Format(s)

Microfluidic biosensing of viscoelastic properties of normal and cancerous human breast cells. / Hu, Shuhuan; Yang, Chengpeng; Hu, Dinglong; Lam, Raymond H. W.

2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems (NEMS). IEEE, 2017. p. 90-95 8016981.

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review