Microbial selenite reduction coupled to anaerobic oxidation of methane

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

10 Scopus Citations
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Author(s)

  • Xiu-Ning Wang
  • Yong-Ze Lu
  • Ling Fu
  • Fang Zhang
  • Raymond J. Zeng

Detail(s)

Original languageEnglish
Pages (from-to)168-174
Journal / PublicationScience of the Total Environment
Volume669
Online published9 Mar 2019
Publication statusPublished - 15 Jun 2019

Abstract

Denitrifying anaerobic methane oxidation (DAMO) is the process of coupling the anaerobic oxidation of methane (AOM) with denitrification, which plays an important part in controlling the flow of methane in anoxic niches. In this study, we explored the feasibility of microbial selenite reduction using methane by DAMO culture. Isotopic 13 CH 4 and long-term experiments showed that selenite reduction was coupled to methane oxidation, and selenite was ultimately reduced to Se (0) by the analyses of scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). The introduction of nitrate, the original electron acceptor in the DAMO culture, inhibited selenite reduction. Meanwhile, the microbial community of DAMO culture was significantly changed when the electron acceptor was changed from nitrate to selenite after long-term selenite reduction. High-throughput 16S rRNA gene sequencing indicated that Methylococcus (26%) became the predominant microbe performing selenite reduction and methane oxidation and the possible pathways of AOM accompanied with selenite reduction were proposed. This study revealed more potential relation during the biogeochemical cycle of carbon, nitrogen, and selenium.

Research Area(s)

  • DAMO, Methane oxidation, Microbial community, Nitrate, Selenite reduction

Citation Format(s)

Microbial selenite reduction coupled to anaerobic oxidation of methane. / Bai, Ya-Nan; Wang, Xiu-Ning; Lu, Yong-Ze; Fu, Ling; Zhang, Fang; Lau, Tai-Chu; Zeng, Raymond J.

In: Science of the Total Environment, Vol. 669, 15.06.2019, p. 168-174.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review