Methyltransferase Domain-Focused Genome Mining for Fungal Polyketide Synthases
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Author(s)
Related Research Unit(s)
Detail(s)
| Original language | English |
|---|---|
| Article number | 2400107 |
| Journal / Publication | Small Methods |
| Volume | 8 |
| Issue number | 11 |
| Online published | 21 Apr 2024 |
| Publication status | Published - 20 Nov 2024 |
Link(s)
| DOI | DOI |
|---|---|
| Attachment(s) | Documents
Publisher's Copyright Statement
|
| Link to Scopus | https://www.scopus.com/record/display.uri?eid=2-s2.0-85190827573&origin=recordpage |
| Permanent Link | https://scholars.cityu.edu.hk/en/publications/publication(cbc057bf-0d4a-44c0-8370-30e7b7403dcf).html |
Abstract
A comparison of substrate-binding site amino acid residues in the C-methyltransferase (MT) domains of fungal nonreducing polyketide synthases (NR-PKSs) suggests that these residues are correlated with the methylation modes used by the PKSs. A PKS, designated as AsbPKS, with substrate-binding site residues distinct from those of other known PKSs is focused on. The characterization of AsbPKS revealed that it yields an isocoumarin derivative, anhydrosclerotinin B (1), the biosynthesis of which involves a previously unreported methylation pattern. This study demonstrates the utility of MT domain-focused genome mining for the discovery of PKSs with new functions. © 2024 The Authors. Small Methods published by Wiley-VCH GmbH.
Research Area(s)
- biosynthesis, methyltransferase domains, natural products, polyketide synthases
Citation Format(s)
Methyltransferase Domain-Focused Genome Mining for Fungal Polyketide Synthases. / Yan, Dexiu; Matsuda, Yudai.
In: Small Methods, Vol. 8, No. 11, 2400107, 20.11.2024.
In: Small Methods, Vol. 8, No. 11, 2400107, 20.11.2024.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
