Metal-Organic Framework Nanoparticle-Assisted Cryopreservation of Red Blood Cells

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review

View graph of relations

Author(s)

  • Wei Zhu
  • Jimin Guo
  • Jacob Ongudi Agola
  • Jonas G. Croissant
  • Zihao Wang
  • Eric Coker
  • Benyamin Motevalli
  • Andreas Zimpel
  • Stefan Wuttke
  • C. Jeffrey Brinker

Related Research Unit(s)

Detail(s)

Original languageEnglish
Pages (from-to)7789-7796
Journal / PublicationJournal of the American Chemical Society
Volume141
Issue number19
Early online date23 Apr 2019
Publication statusPublished - 15 May 2019

Abstract

The development of hybrid nanomaterials mimicking antifreeze proteins that can modulate/inhibit the growth of ice crystals for cell/tissue cryopreservation has attracted increasing interests. Herein, we describe the first utilization of zirconium (Zr)-based metal-organic framework (MOF) nanoparticles (NPs) with well-defined surface chemistries for the cryopreservation of red blood cells (RBCs) without the need of any (toxic) organic solvents. Distinguishing features of this cryoprotective approach include the exceptional water stability, low hemolytic activity, and the long periodic arrangement of organic linkers on the surface of MOF NPs, which provide a precise spacing of hydrogen donors to recognize and match the ice crystal planes. Five kinds of Zr-based MOF NPs, with different pore size, surface chemistry, and framework topologies, were used for the cryoprotection of RBCs. A "splat" assay confirmed that MOF NPs not only exhibited ice recrystallization inhibition activities but also acted as a "catalyst" to accelerate the melting of ice crystals. The human RBC cryopreservation tests displayed RBC recoveries of up to ∼40%, which is higher than that obtained via commonly used hydroxyethyl starch polymers. This cryopreservation approach will inspire the design and utilization of MOF-derived nanoarchitectures for the effective cryopreservation of various cell types as well as tissue samples.

Citation Format(s)

Metal-Organic Framework Nanoparticle-Assisted Cryopreservation of Red Blood Cells. / Zhu, Wei; Guo, Jimin; Agola, Jacob Ongudi; Croissant, Jonas G.; Wang, Zihao; Shang, Jin; Coker, Eric; Motevalli, Benyamin; Zimpel, Andreas; Wuttke, Stefan; Brinker, C. Jeffrey.

In: Journal of the American Chemical Society, Vol. 141, No. 19, 15.05.2019, p. 7789-7796.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review