Meta-lens light-sheet fluorescence microscopy for in vivo imaging

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

20 Scopus Citations
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Author(s)

  • Yuan Luo
  • Ming Lun Tseng
  • Sunil Vyas
  • Ting-Yu Hsieh
  • Jui-Ching Wu
  • Shang-Yang Chen
  • Hsiao-Fang Peng
  • Vin-Cent Su
  • Tzu-Ting Huang
  • Hsin Yu Kuo
  • Cheng Hung Chu
  • Jia-Wern Chen
  • Yu-Chun Chen
  • Kuang-Yuh Huang
  • Chieh-Hsiung Kuan
  • Xu Shi
  • Hiroaki Misawa

Related Research Unit(s)

Detail(s)

Original languageEnglish
Pages (from-to)1949–1959
Number of pages11
Journal / PublicationNanophotonics
Volume11
Issue number9
Online published21 Feb 2022
Publication statusPublished - Apr 2022

Link(s)

Abstract

Light-sheet fluorescent microscopy has become the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. However, designing proper illumination for high image resolution and optical sectioning is challenging. Another issue is geometric constraints arising from the multiple bulky components for illumination and detection. Here, we demonstrate that those issues can be well addressed by integrating nanophotonic meta-lens as the illumination component for LSFM. The meta-lens is composed of 800-nm-thick GaN nanostructures and is designed for a light-sheet well-adapted to biological specimens such as the nematode Caenorhabditis elegans (C. elegans). With the meta-lens, the complexity of the LSFM system is significantly reduced, and it is capable of performing multicolor fluorescent imaging of live C. elegans with cellular resolution. Considering the miniature size and plane geometry of the meta-lens, our system enables a new design for LSFM to acquire in vivo images of biological specimens with high resolution.

Research Area(s)

  • fluorescence microscopy, light sheet microscopy, metasurface, ELEGANS, OPTICS, MODEL, COLOR

Citation Format(s)

Meta-lens light-sheet fluorescence microscopy for in vivo imaging. / Luo, Yuan; Tseng, Ming Lun; Vyas, Sunil et al.
In: Nanophotonics, Vol. 11, No. 9, 04.2022, p. 1949–1959.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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