Metabolic Labeling of Pseudaminic Acid-Containing Glycans on Bacterial Surfaces

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

11 Scopus Citations
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Author(s)

  • Gloria Andolina
  • Ruohan Wei
  • Han Liu
  • Qing Zhang
  • Xuemei Yang
  • Huiluo Cao
  • Aixin Yan
  • Xiang David Li
  • Xuechen Li

Detail(s)

Original languageEnglish
Pages (from-to)3030-3037
Journal / PublicationACS Chemical Biology
Volume13
Issue number10
Online published19 Sep 2018
Publication statusPublished - 19 Oct 2018
Externally publishedYes

Abstract

The rise in antibiotic-resistant bacteria is causing worldwide concerns. The urgent need for new antibacterial drugs calls for new thinking and strategies to explore novel, narrow-spectrum, and pathogen-specific antibacterial targets. Legionaminic acid (Leg) and pseudaminic acid (Pse) are nonulosonic acid carbohydrates with structural similarity to eukaryotic sialic acid, and are distributed in numerous pathogenic Gram-negative bacteria as components of cell surface-associated glycans. They are involved in the host interaction, pathogenicity, antiphage defense mechanism, and immune escape mechanism. To further explore their biological significance, we developed a synthesis of 2-acetamido-4-azidoacetamido-2,4,6-trideoxy-l-altrose (Alt-4NAz) and 2-azidoacetamido-4-acetamido-2,4,6-trideoxy-l-altrose (Alt-2NAz), among which Alt-4NAz served as an effective chemical reporter to realize bacterial Pse metabolic labeling. The effectiveness of this chemical reporter has been demonstrated in Pseudomonas aeruginosa, Vibrio vulnificus, and Acinetobacter baumannii strains. Expectedly, this strategy can provide a useful assay to detect phenotypic presence of Pse biosynthesis and screen for agents targeting this pathway.

Citation Format(s)

Metabolic Labeling of Pseudaminic Acid-Containing Glycans on Bacterial Surfaces. / Andolina, Gloria; Wei, Ruohan; Liu, Han; Zhang, Qing; Yang, Xuemei; Cao, Huiluo; Chen, Sheng; Yan, Aixin; Li, Xiang David; Li, Xuechen.

In: ACS Chemical Biology, Vol. 13, No. 10, 19.10.2018, p. 3030-3037.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review