Meta-lens light-sheet fluorescence microscopy for in vivo imaging

Yuan Luo; Ming Lun Tseng; Sunil Vyas; Ting-Yu Hsieh; Jui-Ching Wu; Shang-Yang Chen; Hsiao-Fang Peng; Vin-Cent Su; Tzu-Ting Huang; Hsin Yu Kuo; Cheng Hung Chu; Mu Ku Chen; Jia-Wern Chen; Yu-Chun Chen; Kuang-Yuh Huang; Chieh-Hsiung Kuan; Xu Shi; Hiroaki Misawa; Din Ping Tsai

doi:10.1515/nanoph-2021-0748

Meta-lens light-sheet fluorescence microscopy for in vivo imaging

Yuan Luo^* (Co-first Author)
, Ming Lun Tseng (Co-first Author)
, Sunil Vyas (Co-first Author)
, Ting-Yu Hsieh
, Jui-Ching Wu^*
, Shang-Yang Chen
, Hsiao-Fang Peng
, Vin-Cent Su
, Tzu-Ting Huang
, Hsin Yu Kuo
, Cheng Hung Chu
, Mu Ku Chen
, Jia-Wern Chen
, Yu-Chun Chen
, Kuang-Yuh Huang
, Chieh-Hsiung Kuan
, Xu Shi
, Hiroaki Misawa
, Din Ping Tsai^*

^*Corresponding author for this work

Department of Electrical Engineering

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

54 Citations (Scopus)

111 Downloads (CityUHK Scholars)

Abstract

Light-sheet fluorescent microscopy has become the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. However, designing proper illumination for high image resolution and optical sectioning is challenging. Another issue is geometric constraints arising from the multiple bulky components for illumination and detection. Here, we demonstrate that those issues can be well addressed by integrating nanophotonic meta-lens as the illumination component for LSFM. The meta-lens is composed of 800-nm-thick GaN nanostructures and is designed for a light-sheet well-adapted to biological specimens such as the nematode Caenorhabditis elegans (C. elegans). With the meta-lens, the complexity of the LSFM system is significantly reduced, and it is capable of performing multicolor fluorescent imaging of live C. elegans with cellular resolution. Considering the miniature size and plane geometry of the meta-lens, our system enables a new design for LSFM to acquire in vivo images of biological specimens with high resolution.

Original language	English
Pages (from-to)	1949–1959
Number of pages	11
Journal	Nanophotonics
Volume	11
Issue number	9
Online published	21 Feb 2022
DOIs	https://doi.org/10.1515/nanoph-2021-0748
Publication status	Published - Apr 2022

Funding

The authors acknowledge financial support from the Ministry of Science and Technology, Taiwan (Grant No. 108-2221-E-002-168-MY4), and National Taiwan University (NTU-107L7728, NTU-107L7807, and NTU-YIH-08HZT49001. They are also grateful to the Shenzhen Science and Technology Innovation Commission Grant (No. SGDX2019081623281169), the University Grants Committee/Research Grants Council of theHong Kong Special Administrative Region, China (Project No. AoE/P-502/20 and GRF Project: 15303521), the Department of Science and Technology of Guangdong Province (2020B1515120073), and the Department of Electrical Engineering, City University of Hong Kong (Project No. 9380131). X. S. and H. M. acknowledge financial support from JSPS KAKENHI Grant No. JP18H05205, the Nanotechnology Platform (Hokkaido University), and the Dynamic Alliance for Open Innovation Bridging Human, Environment, and Materials (Five-Star Alliance) of MEXT.

Research Keywords

fluorescence microscopy
light sheet microscopy
metasurface
ELEGANS
OPTICS
MODEL
COLOR

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This full text is made available under CC-BY 4.0. https://creativecommons.org/licenses/by/4.0/

RGC Funding Information

RGC-funded

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10.1515/nanoph-2021-0748

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Luo, Y., Tseng, M. L., Vyas, S., Hsieh, T.-Y., Wu, J.-C., Chen, S.-Y., Peng, H.-F., Su, V.-C., Huang, T.-T., Kuo, H. Y., Chu, C. H., Chen, M. K., Chen, J.-W., Chen, Y.-C., Huang, K.-Y., Kuan, C.-H., Shi, X., Misawa, H., & Tsai, D. P. (2022). Meta-lens light-sheet fluorescence microscopy for in vivo imaging. Nanophotonics, 11(9), 1949–1959. https://doi.org/10.1515/nanoph-2021-0748

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abstract = "Light-sheet fluorescent microscopy has become the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. However, designing proper illumination for high image resolution and optical sectioning is challenging. Another issue is geometric constraints arising from the multiple bulky components for illumination and detection. Here, we demonstrate that those issues can be well addressed by integrating nanophotonic meta-lens as the illumination component for LSFM. The meta-lens is composed of 800-nm-thick GaN nanostructures and is designed for a light-sheet well-adapted to biological specimens such as the nematode Caenorhabditis elegans (C. elegans). With the meta-lens, the complexity of the LSFM system is significantly reduced, and it is capable of performing multicolor fluorescent imaging of live C. elegans with cellular resolution. Considering the miniature size and plane geometry of the meta-lens, our system enables a new design for LSFM to acquire in vivo images of biological specimens with high resolution.",

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Meta-lens light-sheet fluorescence microscopy for in vivo imaging

Abstract

Funding

Research Keywords

Publisher's Copyright Statement

RGC Funding Information

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AoE(UGC): Meta-optics, Meta-acoustics and Meta-device

GRF: Bound States in the Continuum Enhanced Second Harmonic Generation of Lithium Niobate

SZSTIB-C-HK: 全介質寬色域、高飽和度、高亮度和亞波長分辨率結構色的研究

Cite this

Meta-lens light-sheet fluorescence microscopy for in vivo imaging

Abstract

Funding

Research Keywords

Publisher's Copyright Statement

RGC Funding Information

Access Output

Other Access Options

Permanent Link

Other Files and Links

Fingerprint

Projects

AoE(UGC): Meta-optics, Meta-acoustics and Meta-device

GRF: Bound States in the Continuum Enhanced Second Harmonic Generation of Lithium Niobate

SZSTIB-C-HK: 全介質寬色域、高飽和度、高亮度和亞波長分辨率結構色的研究

Cite this