Mesalamine-Mediated Amelioration of Experimental Colitis in Piglets Involves Gut Microbiota Modulation and Intestinal Immune Cell Infiltration

Yonggang Huang; Miaomiao Wu; Hao Xiao; Hongnan Liu; Guan Yang

doi:10.3389/fimmu.2022.883682

Mesalamine-Mediated Amelioration of Experimental Colitis in Piglets Involves Gut Microbiota Modulation and Intestinal Immune Cell Infiltration

Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review

Overview

1 Scopus Citations

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Author(s)

Yonggang Huang
Miaomiao Wu
Hao Xiao
Hongnan Liu
Guan Yang

Related Research Unit(s)

Department of Infectious Diseases and Public Health

Detail(s)

Original language	English
Article number	883682
Journal / Publication	Frontiers in Immunology
Volume	13
Online published	8 Jul 2022
Publication status	Published - Jul 2022

Link(s)

DOI	DOI https://doi.org/10.3389/fimmu.2022.883682 Final Published version
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Attachment(s)	Documents 110947012.pdf Final Published version, 9.73 MB, PDF Publisher's Copyright Statement This full text is made available under CC-BY 4.0. https://creativecommons.org/licenses/by/4.0/
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-85134611676&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(162a9fd2-9bfc-4362-83bc-a54ab9346fab).html

Abstract

Mesalamine (MES), also known as 5-aminosalicylic acid, is effective in treating mild to moderate ulcerative colitis (UC). The mechanisms of its actions are not fully elucidated. The aim of this study was to investigate the effects of MES treatment on intestinal microbiota and immune system in an dextran sulfate sodium (DSS)-induced UC model in postweaning piglets. Eighteen weaned piglets were assigned randomly to the following treatments: control group (CON, distilled water), DSS group (DSS, 3% DSS), and MES group (MES, 3% DSS + 2 g/day MES). Our results showed that MES treatment alleviates DSS-induced colitis in piglets, as evidenced by a reduced diarrhea index score and increased average daily gain (P < 0.05). This is accompanied by decreased diamine oxidase activity, D-lactate level (P < 0.05), and attenuated mucosal damage. MES treatment also decreased the abundance of Methanogens and reduced colon CD11b⁺ macrophage and CD3⁺ T-cell infiltrations in piglets with DSS-induced colitis (P < 0.05). Collectively, these data indicate that MES treatment-mediated colitis protection may involve microbiota and immune cell alterations.