Mechanistic study of transcription factor Sox18 during heart development

Jianxin Liang; Imtiaz Ul Hassan; Man Yee Cheung; Lei Feng; Yi-jyun Lin; Qi Long; Chengdong Wang; Yuyue Ding; Ziqing Wang; Yuan Zhang; Yulong Li; Donghao Guo; Xiaofang Guo; Thomas Chi Bun Wong; Muhammad Kaleem Samma; Zixin Rong; Xufeng Qi; Dongqing Cai; Sai-Ming Ngai; Hui Zhao

doi:10.1016/j.ygcen.2024.114472

Mechanistic study of transcription factor Sox18 during heart development

Jianxin Liang, Imtiaz Ul Hassan, Man Yee Cheung, Lei Feng, Yi-jyun Lin, Qi Long, Chengdong Wang, Yuyue Ding, Ziqing Wang, Yuan Zhang, Yulong Li, Donghao Guo, Xiaofang Guo, Thomas Chi Bun Wong, Muhammad Kaleem Samma, Zixin Rong, Xufeng Qi, Dongqing Cai, Sai-Ming Ngai, Hui Zhao^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

1 Citation (Scopus)

Abstract

Heart development is a delicate and complex process regulated by coordination of various signaling pathways. In this study, we investigated the role of sox18 in heart development by modulating Wnt/β-Catenin signaling pathways. Our spatiotemporal expression analysis revealed that sox18 is mainly expressed in the heart, branchial arch, pharyngeal arch, spinal cord, and intersegmental vessels at the tailbud stage of Xenopus tropicalis embryo. Overexpression of sox18 in the X. tropicalis embryos causes heart edema, while loss-of-function of sox18 can change the signal of developmental heart marker gata4 at different stages, suggesting that sox18 plays an essential role in the development of the heart. Knockdown of SOX18 in human umbilical vein endothelial cells suggests a link between Sox18 and β-CATENIN, a key regulator of the Wnt signaling pathway. Sox18 negatively regulates islet1 and tbx3, the downstream factors of Wnt/β-Catenin signaling, during the linear heart tube formation and the heart looping stage. Taken together, our findings highlight the crucial role of Sox18 in the development of the heart via inhibiting Wnt/β-Catenin signaling. © 2024 Elsevier Inc.

Original language	English
Article number	114472
Journal	General and Comparative Endocrinology
Volume	350
Online published	17 Feb 2024
DOIs	https://doi.org/10.1016/j.ygcen.2024.114472
Publication status	Published - 1 May 2024

Funding

This work was supported by the National Key R&D Program of China, Grant/Award Number: 2016YFE0204700 , the Research Grants Council of Hong Kong (14112618 and 14119120) to HZ, and CRF equipment grant C5033-19E. Additional support was provided by the Hong Kong Branch of CAS Center for Excellence in Animal Evolution and Genetics to HZ (8601012). We thank our laboratory colleagues for their helpful discussion in this project.

Research Keywords

Heart development
Sox18
Transcriptional factor
Wnt signaling pathway
Xenopus

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RGC-funded

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10.1016/j.ygcen.2024.114472

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Liang, J., Ul Hassan, I., Yee Cheung, M., Feng, L., Lin, Y., Long, Q., Wang, C., Ding, Y., Wang, Z., Zhang, Y., Li, Y., Guo, D., Guo, X., Chi Bun Wong, T., Kaleem Samma, M., Rong, Z., Qi, X., Cai, D., Ngai, S.-M., & Zhao, H. (2024). Mechanistic study of transcription factor Sox18 during heart development. General and Comparative Endocrinology, 350, Article 114472. https://doi.org/10.1016/j.ygcen.2024.114472

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title = "Mechanistic study of transcription factor Sox18 during heart development",

abstract = "Heart development is a delicate and complex process regulated by coordination of various signaling pathways. In this study, we investigated the role of sox18 in heart development by modulating Wnt/β-Catenin signaling pathways. Our spatiotemporal expression analysis revealed that sox18 is mainly expressed in the heart, branchial arch, pharyngeal arch, spinal cord, and intersegmental vessels at the tailbud stage of Xenopus tropicalis embryo. Overexpression of sox18 in the X. tropicalis embryos causes heart edema, while loss-of-function of sox18 can change the signal of developmental heart marker gata4 at different stages, suggesting that sox18 plays an essential role in the development of the heart. Knockdown of SOX18 in human umbilical vein endothelial cells suggests a link between Sox18 and β-CATENIN, a key regulator of the Wnt signaling pathway. Sox18 negatively regulates islet1 and tbx3, the downstream factors of Wnt/β-Catenin signaling, during the linear heart tube formation and the heart looping stage. Taken together, our findings highlight the crucial role of Sox18 in the development of the heart via inhibiting Wnt/β-Catenin signaling. {\textcopyright} 2024 Elsevier Inc.",

keywords = "Heart development, Sox18, Transcriptional factor, Wnt signaling pathway, Xenopus",

author = "Jianxin Liang and {Ul Hassan}, Imtiaz and {Yee Cheung}, Man and Lei Feng and Yi-jyun Lin and Qi Long and Chengdong Wang and Yuyue Ding and Ziqing Wang and Yuan Zhang and Yulong Li and Donghao Guo and Xiaofang Guo and {Chi Bun Wong}, Thomas and {Kaleem Samma}, Muhammad and Zixin Rong and Xufeng Qi and Dongqing Cai and Sai-Ming Ngai and Hui Zhao",

year = "2024",

month = may,

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doi = "10.1016/j.ygcen.2024.114472",

language = "English",

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journal = "General and Comparative Endocrinology",

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Liang, J, Ul Hassan, I, Yee Cheung, M, Feng, L, Lin, Y, Long, Q, Wang, C, Ding, Y, Wang, Z, Zhang, Y, Li, Y, Guo, D, Guo, X, Chi Bun Wong, T, Kaleem Samma, M, Rong, Z, Qi, X, Cai, D, Ngai, S-M & Zhao, H 2024, 'Mechanistic study of transcription factor Sox18 during heart development', General and Comparative Endocrinology, vol. 350, 114472. https://doi.org/10.1016/j.ygcen.2024.114472

TY - JOUR

T1 - Mechanistic study of transcription factor Sox18 during heart development

AU - Liang, Jianxin

AU - Ul Hassan, Imtiaz

AU - Yee Cheung, Man

AU - Feng, Lei

AU - Lin, Yi-jyun

AU - Long, Qi

AU - Wang, Chengdong

AU - Ding, Yuyue

AU - Wang, Ziqing

AU - Zhang, Yuan

AU - Li, Yulong

AU - Guo, Donghao

AU - Guo, Xiaofang

AU - Chi Bun Wong, Thomas

AU - Kaleem Samma, Muhammad

AU - Rong, Zixin

AU - Qi, Xufeng

AU - Cai, Dongqing

AU - Ngai, Sai-Ming

AU - Zhao, Hui

PY - 2024/5/1

Y1 - 2024/5/1

N2 - Heart development is a delicate and complex process regulated by coordination of various signaling pathways. In this study, we investigated the role of sox18 in heart development by modulating Wnt/β-Catenin signaling pathways. Our spatiotemporal expression analysis revealed that sox18 is mainly expressed in the heart, branchial arch, pharyngeal arch, spinal cord, and intersegmental vessels at the tailbud stage of Xenopus tropicalis embryo. Overexpression of sox18 in the X. tropicalis embryos causes heart edema, while loss-of-function of sox18 can change the signal of developmental heart marker gata4 at different stages, suggesting that sox18 plays an essential role in the development of the heart. Knockdown of SOX18 in human umbilical vein endothelial cells suggests a link between Sox18 and β-CATENIN, a key regulator of the Wnt signaling pathway. Sox18 negatively regulates islet1 and tbx3, the downstream factors of Wnt/β-Catenin signaling, during the linear heart tube formation and the heart looping stage. Taken together, our findings highlight the crucial role of Sox18 in the development of the heart via inhibiting Wnt/β-Catenin signaling. © 2024 Elsevier Inc.

AB - Heart development is a delicate and complex process regulated by coordination of various signaling pathways. In this study, we investigated the role of sox18 in heart development by modulating Wnt/β-Catenin signaling pathways. Our spatiotemporal expression analysis revealed that sox18 is mainly expressed in the heart, branchial arch, pharyngeal arch, spinal cord, and intersegmental vessels at the tailbud stage of Xenopus tropicalis embryo. Overexpression of sox18 in the X. tropicalis embryos causes heart edema, while loss-of-function of sox18 can change the signal of developmental heart marker gata4 at different stages, suggesting that sox18 plays an essential role in the development of the heart. Knockdown of SOX18 in human umbilical vein endothelial cells suggests a link between Sox18 and β-CATENIN, a key regulator of the Wnt signaling pathway. Sox18 negatively regulates islet1 and tbx3, the downstream factors of Wnt/β-Catenin signaling, during the linear heart tube formation and the heart looping stage. Taken together, our findings highlight the crucial role of Sox18 in the development of the heart via inhibiting Wnt/β-Catenin signaling. © 2024 Elsevier Inc.

KW - Heart development

KW - Sox18

KW - Transcriptional factor

KW - Wnt signaling pathway

KW - Xenopus

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UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85186741957&origin=recordpage

U2 - 10.1016/j.ygcen.2024.114472

DO - 10.1016/j.ygcen.2024.114472

M3 - RGC 21 - Publication in refereed journal

C2 - 38373462

SN - 0016-6480

VL - 350

JO - General and Comparative Endocrinology

JF - General and Comparative Endocrinology

M1 - 114472

ER -

Mechanistic study of transcription factor Sox18 during heart development

Abstract

Funding

Research Keywords

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