Measurement of alpha radiation based on amperometric activity of bacteria focused electrokinectically in microfluidics

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)

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Detail(s)

Original languageEnglish
Title of host publication2016 IEEE 10th International Conference on Nano/Molecular Medicine and Engineering (NANOMED)
PublisherIEEE
Pages93-96
ISBN (Electronic)978-1-5090-4675-1
ISBN (Print)978-1-5090-4676-8
Publication statusPublished - Oct 2016

Publication series

Name
ISSN (Print)2159-6964
ISSN (Electronic)2159-6972

Conference

TitleIEEE-NANOMED 2016
PlaceChina
CityMacau
Period30 October - 2 November 2016

Abstract

The nuclear radiation exposure in Fukushima in 2011 has aroused the publics' anxiety on nuclear safety. The alpha particles in such radiation may induce cancer and other serious diseases once entering the human body, yet high-sensitivity detection of alpha radiation remains unavailable and technically challenging. Here, we report the development of an effective and reliable alpha radiation measurement scheme requiring an exposure area of <1 cm2 based on microfluidics. The working principle is based on the β-galactosidase generation of genetically modified radiation-resistant bacteria Deinococcus Radiodurans upon exposure of alpha radiation. We further adopt dielectrophoretic focusing to increase the local population density of D. Radiodurans in the exposure region in the device. P-Aminophenyl-β-D-galacto-pyranoside (PAPG) will be added to the detection chamber in order to convert β-galactosidase to p-Aminophenol (PAP), which can then be quantified by amperometry through electrochemical sensing electrodes prefabricated inside the detection chamber. Therefore, the microfluidic radiation sensor can quantify the alpha radiation level as electrical signals. As a pilot study, we first apply isopropyl β-D-1-Thiogalactopyranoside (IPTG) to induce an equivalent effect as the radiation exposure for calibrating the microfluidic detection scheme. We will present our results on the measurement of alpha radiation in our conference presentation. Altogether, this work has demonstrated a novel biomicrofluidic technique for achieving effective and reliable radiation monitoring with reduced cost.

Citation Format(s)

Measurement of alpha radiation based on amperometric activity of bacteria focused electrokinectically in microfluidics. / Yang, Chengpeng; Li, Vincent W.T.; Yu, Peter K.N.; Cheng, S. H.; Lam, Raymond H.W.

2016 IEEE 10th International Conference on Nano/Molecular Medicine and Engineering (NANOMED). IEEE, 2016. p. 93-96 7883573.

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)