Major human Hepatitis A virus genotype in Hong Kong marine waters and detection by real-time PCR

Ning Yang; Daniel Ling Ho Chu; Minnie Man Lai Wong; Huizhou Qi; Rudolf Shiu Sun Wu; Richard Yuen Chong Kong

doi:10.1016/j.marpolbul.2011.09.027

Author(s)

Ning Yang
Daniel Ling Ho Chu
Minnie Man Lai Wong
Huizhou Qi
Rudolf Shiu Sun Wu

Related Research Unit(s)

State Key Laboratory of Marine Pollution (City University of Hong Kong)

Detail(s)

Original language	English
Pages (from-to)	2654-2658
Journal / Publication	Marine Pollution Bulletin
Volume	62
Issue number	12
Publication status	Published - Dec 2011

Link(s)

DOI	DOI https://doi.org/10.1016/j.marpolbul.2011.09.027 Final Published version
	Check@CityULib
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-81855194308&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(97d558c9-d7d1-4067-b42b-1e6a0f5210b9).html

Abstract

Marine waters from seven sites around Hong Kong with varying levels of sewage pollution were analyzed for Hepatitis A virus (HAV) by PCR cloning and DNA sequencing of the highly variable VP1/2A junction of the HAV genome. Phylogenetic analysis of 10 PCR clones from each of the HAV-positive marine sites indicated that human HAV genotype IB is the most widely distributed type in Hong Kong waters. A sensitive and quantitative TaqMan-based PCR method targeting the 5'-noncoding region (5'-NCR) of HAV was used to quantify HAV particles in marine water samples along with the total Escherichia coli counts being enumerated on TBX medium for comparison. Our results showed that no correlation of any significance between HAV and E. coli counts was observed which underscores the inadequacy in using E. coli as a sanitary standard to predict the levels of HAV in marine waters. © 2011 Elsevier Ltd.

Research Area(s)

Fecal contamination, HAV genotype IB, Hepatitis A virus, Hong Kong marine waters, TaqMan real-time PCR

Citation Format(s)

[ RIS ] [ BibTeX ]

Major human Hepatitis A virus genotype in Hong Kong marine waters and detection by real-time PCR. / Yang, Ning; Chu, Daniel Ling Ho; Wong, Minnie Man Lai et al.
In: Marine Pollution Bulletin, Vol. 62, No. 12, 12.2011, p. 2654-2658.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

Yang, N, Chu, DLH, Wong, MML, Qi, H, Wu, RSS & Kong, RYC 2011, 'Major human Hepatitis A virus genotype in Hong Kong marine waters and detection by real-time PCR', Marine Pollution Bulletin, vol. 62, no. 12, pp. 2654-2658. https://doi.org/10.1016/j.marpolbul.2011.09.027

Yang, N., Chu, D. L. H., Wong, M. M. L., Qi, H., Wu, R. S. S., & Kong, R. Y. C. (2011). Major human Hepatitis A virus genotype in Hong Kong marine waters and detection by real-time PCR. Marine Pollution Bulletin, 62(12), 2654-2658. https://doi.org/10.1016/j.marpolbul.2011.09.027

Yang N, Chu DLH, Wong MML, Qi H, Wu RSS, Kong RYC. Major human Hepatitis A virus genotype in Hong Kong marine waters and detection by real-time PCR. Marine Pollution Bulletin. 2011 Dec;62(12):2654-2658. doi: 10.1016/j.marpolbul.2011.09.027

Yang, Ning ; Chu, Daniel Ling Ho ; Wong, Minnie Man Lai et al. / Major human Hepatitis A virus genotype in Hong Kong marine waters and detection by real-time PCR. In: Marine Pollution Bulletin. 2011 ; Vol. 62, No. 12. pp. 2654-2658.