Magnetization transfer contrast MRI for non-invasive assessment of innate and adaptive immune responses against alginate-encapsulated cells

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

11 Scopus Citations
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Author(s)

Detail(s)

Original languageEnglish
Pages (from-to)7811-7818
Journal / PublicationBiomaterials
Volume35
Issue number27
Online published13 Jun 2014
Publication statusPublished - Sep 2014
Externally publishedYes

Abstract

By means of physical isolation of cells inside semi-permeable hydrogels, encapsulation has been widely used to immunoprotect transplanted cells. While spherical alginate microcapsules are now being used clinically, there still is little known about the patient's immune system response unless biopsies are obtained. We investigated the use of Magnetization Transfer (MT) imaging to non-invasively detect host immune responses against alginate capsules containing xenografted human hepatocytes in four groups of animals, including transplanted empty capsules (-Cells/-IS), capsules with live cells with (+LiveCells/+IS) and without immunosuppression (+LiveCells/-IS), and capsules with apoptotic cells in non-immunosuppressed animals (+DeadCells/-IS). The highest MT ratio (MTR) was found in+LiveCells/-IS, which increased from day 0 by 38% and 53% on days 7 and 14 after transplantation respectively, and corresponded to a distinctive increase in cell infiltration on histology. Furthermore, we show that macromolecular ratio maps based on MT data are more sensitive to cell infiltration and fibrosis than conventional MTR maps. Such maps showed a significant difference between+LiveCells/-IS (0.18±0.02) and+DeadCells/-IS (0.13±0.02) on day 7 (P

Research Area(s)

  • Alginate microcapsules, Cellular imaging, Immune response, Molecular imaging, MRI

Citation Format(s)

Magnetization transfer contrast MRI for non-invasive assessment of innate and adaptive immune responses against alginate-encapsulated cells. / Chan, Kannie W.Y.; Liu, Guanshu; van Zijl, Peter C.M.; Bulte, Jeff W.M.; McMahon, Michael T.

In: Biomaterials, Vol. 35, No. 27, 09.2014, p. 7811-7818.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal