Magnetic-Particle-Encapsulated Alginate Beads for Aqueous-Based Bacteria Culturing and Manipulation

Shuai Yuan; P. T. Lai; Anderson H. C. Shum; Philip W. T. Pong

doi:10.1109/TMAG.2021.3102959

Magnetic-Particle-Encapsulated Alginate Beads for Aqueous-Based Bacteria Culturing and Manipulation

Shuai Yuan
, P. T. Lai
, Anderson H. C. Shum^*
, Philip W. T. Pong^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

2 Citations (Scopus)

Abstract

Active sorting of bacteria has shown great potential in revealing the huge biodiversity of various microbial communities. Droplet microfluidics offer a powerful tool for bacteria culturing and sorting. However, most traditional sorting methods fail to work in all-aqueous droplet microfluidic systems, due to the similar dielectric constants between aqueous solutions. This drawback hinders the application of droplet microfluidic in bacteria screening. Magnetic manipulation is a good candidate to overcome this hurdle, since it can provide relatively long-range, non-contact force regardless of the dielectric properties of the solutions. In this article, Fe3O4 magnetic nanoparticles (Fe₃O₄MNPs) encapsulated alginate beads containing bacteria were generated. The capsules can be manipulated using an external magnetic field, making it possible for effective sorting after culturing. The generation of the alginate capsules under different conditions was systematically studied. The experiments showed that the bacteria-laden capsules can be manipulated using magnetic field. Bacteria culturing demonstrated that bacteria can be raised inside the alginate beads, verifying the feasibility of the proposed technique. © 2021 IEEE.

Original language	English
Article number	5200205
Journal	IEEE Transactions on Magnetics
Volume	58
Issue number	2
Online published	6 Aug 2021
DOIs	https://doi.org/10.1109/TMAG.2021.3102959
Publication status	Published - Feb 2022
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Funding

The work of Philip W. T. Pong was supported in part by the Startup Fund from the New Jersey Institute of Technology; in part by the Seed Funding Program for Basic Research, Seed Funding Program for Applied Research and Small Project Funding Program from the University of Hong Kong; in part by the Innovation and Technology Fund (ITF) Tier 3 under Grant ITS/203/14, Grant ITS/104/13, and Grant ITS/214/14; in part by the Research Grants Council-General Research Fund (RGC-GRF) under Grant HKU 17210014 and Grant HKU 17204617; and in part by the University Grants Committee of Hong Kong under Contract AoE/P-04/08

Research Keywords

Bacterium culturing
droplet microfluidics
magnetic manipulation

RGC Funding Information

RGC-funded

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10.1109/TMAG.2021.3102959

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title = "Magnetic-Particle-Encapsulated Alginate Beads for Aqueous-Based Bacteria Culturing and Manipulation",

abstract = "Active sorting of bacteria has shown great potential in revealing the huge biodiversity of various microbial communities. Droplet microfluidics offer a powerful tool for bacteria culturing and sorting. However, most traditional sorting methods fail to work in all-aqueous droplet microfluidic systems, due to the similar dielectric constants between aqueous solutions. This drawback hinders the application of droplet microfluidic in bacteria screening. Magnetic manipulation is a good candidate to overcome this hurdle, since it can provide relatively long-range, non-contact force regardless of the dielectric properties of the solutions. In this article, Fe3O4 magnetic nanoparticles (Fe3O4MNPs) encapsulated alginate beads containing bacteria were generated. The capsules can be manipulated using an external magnetic field, making it possible for effective sorting after culturing. The generation of the alginate capsules under different conditions was systematically studied. The experiments showed that the bacteria-laden capsules can be manipulated using magnetic field. Bacteria culturing demonstrated that bacteria can be raised inside the alginate beads, verifying the feasibility of the proposed technique. {\textcopyright} 2021 IEEE.",

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AU - Yuan, Shuai

AU - Lai, P. T.

AU - Shum, Anderson H. C.

AU - Pong, Philip W. T.

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2022/2

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N2 - Active sorting of bacteria has shown great potential in revealing the huge biodiversity of various microbial communities. Droplet microfluidics offer a powerful tool for bacteria culturing and sorting. However, most traditional sorting methods fail to work in all-aqueous droplet microfluidic systems, due to the similar dielectric constants between aqueous solutions. This drawback hinders the application of droplet microfluidic in bacteria screening. Magnetic manipulation is a good candidate to overcome this hurdle, since it can provide relatively long-range, non-contact force regardless of the dielectric properties of the solutions. In this article, Fe3O4 magnetic nanoparticles (Fe3O4MNPs) encapsulated alginate beads containing bacteria were generated. The capsules can be manipulated using an external magnetic field, making it possible for effective sorting after culturing. The generation of the alginate capsules under different conditions was systematically studied. The experiments showed that the bacteria-laden capsules can be manipulated using magnetic field. Bacteria culturing demonstrated that bacteria can be raised inside the alginate beads, verifying the feasibility of the proposed technique. © 2021 IEEE.

AB - Active sorting of bacteria has shown great potential in revealing the huge biodiversity of various microbial communities. Droplet microfluidics offer a powerful tool for bacteria culturing and sorting. However, most traditional sorting methods fail to work in all-aqueous droplet microfluidic systems, due to the similar dielectric constants between aqueous solutions. This drawback hinders the application of droplet microfluidic in bacteria screening. Magnetic manipulation is a good candidate to overcome this hurdle, since it can provide relatively long-range, non-contact force regardless of the dielectric properties of the solutions. In this article, Fe3O4 magnetic nanoparticles (Fe3O4MNPs) encapsulated alginate beads containing bacteria were generated. The capsules can be manipulated using an external magnetic field, making it possible for effective sorting after culturing. The generation of the alginate capsules under different conditions was systematically studied. The experiments showed that the bacteria-laden capsules can be manipulated using magnetic field. Bacteria culturing demonstrated that bacteria can be raised inside the alginate beads, verifying the feasibility of the proposed technique. © 2021 IEEE.

KW - Bacterium culturing

KW - droplet microfluidics

KW - magnetic manipulation

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DO - 10.1109/TMAG.2021.3102959

M3 - RGC 21 - Publication in refereed journal

SN - 0018-9464

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JO - IEEE Transactions on Magnetics

JF - IEEE Transactions on Magnetics

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ER -

Magnetic-Particle-Encapsulated Alginate Beads for Aqueous-Based Bacteria Culturing and Manipulation

Abstract

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Research Keywords

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